Proteome profiling of the sperm maturation milieu in the rhesus monkey (Macaca mulatta) epididymis
Proteome profiling of the sperm maturation milieu in the rhesus monkey (Macaca mulatta) epididymis
复制标题
恒河猴（Macaca mulatta）附睾精子成熟环境的蛋白质组分析
DOI：
10.1071/rd14322
复制
发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Liu, Fu-Jun
中科院分区：
文献类型：
--
作者：
Liu, Xin;Jin, Shao-Hua;Liu, Fu-Jun
哺乳动物精子在通过附睾的过程中获得其受精潜力，在那里它与附睾腔液蛋白(精子成熟环境)相互作用。为了突出恒河猴(Macaca Mulatta)精子成熟过程中附睾特异的功能，对附睾腔液蛋白质进行了双向凝胶电泳，然后用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF/MS)或MALDI-TOF/TOF进行鉴定，共发现了500多个斑点，由198个非冗余蛋白质组成。部分质谱学数据经Western blotting鉴定得到证实。附睾液中常见的蛋白质有聚集素、α-1-抗胰蛋白酶、苹果酸脱氢酶、L-乳酸脱氢酶B、α-1-酸性糖蛋白1和α-甘露糖苷酶。超过7%的蛋白质是抗氧化的，这可能会控制男性体内的氧化应激。与公牛和人类附睾腔液蛋白相比，恒河猴的这些蛋白与人类有更多的重叠，这为恒河猴和人类之间的密切进化关系提供了证据。本研究为恒河猴附睾功能提供了新的蛋白质组学信息，并为无创评估雄性生育能力提供了新的潜在生物标志物。
The mammalian spermatozoon acquires its fertilising potential during transit through the epididymis, where it interacts with epididymal luminal fluid proteins (the sperm maturation milieu). In order to highlight the epididymal-specific function of the rhesus monkey (Macaca mulatta) in sperm maturation, two-dimensional gel electrophoresis of epididymal luminal fluid proteins was followed by identification by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS) or MALDI-TOF/TOF and revealed over five hundred spots, comprising 198 non-redundant proteins. Some mass spectrometric data were confirmed by western blotting identification. Some common epididymal fluid proteins were identified, such as clusterin, alpha-1-antitrypsin, malate dehydrogenase, L-lactate dehydrogenase B, alpha-1-acid glycoprotein 1 and a-mannosidase. More than 7% of all proteins were anti-oxidative, which might control oxidative stress within the male tract. When compared with bull and human epididymal luminal fluid proteins, those in the rhesus monkey had more overlap with the human, which provides evidence of a close evolutionary relationship between the rhesus monkey and man. This study provides new proteomic information on possible rhesus monkey epididymal functions and novel potential biomarkers for the noninvasive assessment of male fertility.