Effects of four medicinal herbs on human vascular endothelial cells in culture
Effects of four medicinal herbs on human vascular endothelial cells in culture
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DOI：
10.1016/j.ijcard.2007.05.111
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发表时间：
2008-08-29
影响因子：
3.5
通讯作者：
Komesaroff, Paul
中科院分区：
文献类型：
--
作者：
Ling, Shanhong;Nheu, Lina;Komesaroff, Paul
背景资料：丹参（DS，丹参），山赤（SQ，三七），山仔（SZ，山楂）和何首乌（HSW，何首乌）是四种常用的中药，以前被证明具有可能有助于心血管保护的活性。本研究的目的是探讨这些草药对血管内皮细胞的细胞活力和细胞粘附分子的表达炎症conditions.Methods的影响：草药提取物制备了一个既定的工业生产过程。在正常或无血清培养和肿瘤坏死因子（TNF）α刺激下，将人脐静脉内皮细胞（HUVEC）与草药提取物一起孵育。通过形态学检查、Annexin-V染色、Western印迹分析、流式细胞术、[H-3]-胸苷掺入试验和细胞数量研究评估细胞凋亡、凋亡相关基因表达、细胞粘附分子表达、DNA合成和生长。SZ和HSW显著抑制血清剥夺和TNF α刺激的HUVEC凋亡，并伴有caspase-3基因表达下调。DS和SQ显著减弱TNF α诱导的细胞中粘附分子VCAM-1和ICAM-1的表达。在正常培养条件下，4种中草药在治疗浓度（100 μ g/mL）下均抑制HUVEC的DNA合成（[H-3]-胸苷掺入率降低10-42%）和生长（细胞数减少5-10%）。HSW和SZ减少细胞凋亡，DS和SQ抑制粘附分子表达的作用可能有助于保护内皮功能和抑制动脉粥样硬化的形成，而它们抑制DNA合成和细胞生长的作用可能削弱内皮修复的能力。需要进一步的研究，以确定负责这些草药的这些生理作用的化学化合物。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Background: Danshen (DS, Salvia miltiorrhiza), Shanchi (SQ, Panax notoginseng), Shanzai (SZ, Hawthorn) and Heshouwu (HSW, Polygonum multiflorum Thunb) are four medicinal herbs commonly used in traditional Chinese medicine and previously shown to have activity that may contribute to cardiovascular protection. This study aims to investigate effects of these herbs on vascular endothelial cells with respect to cell viability and expression of cellular adhesion molecules under inflammatory conditions.Methods: Herbal extracts were prepared by an established industrial manufacturing process. Human umbilical vein endothelial cells (HUVEC) were incubated with the herbal extract under normal or serum-free culture and tumor necrosis factor (TNF) alpha stimulation. Cell apoptosis, apoptosis-associated gene expression, expression of cellular adhesion molecules, DNA synthesis, and growth were assessed via morphological examination, Annexin-V staining, Western blotting analysis, Flow-Cytometry, [H-3]-thymidine incorporation assay, and cell number study.Results: SZ and HSW significantly inhibited apoptosis in HUVEC undergoing serum deprivation and TNF alpha stimulation, accompanied by down-regulation of caspase-3 gene expression. DS and SQ significantly attenuated TNF alpha-induced expression of adhesion molecule VCAM-1 and also ICAM-1 by DS in the cells. All four herbs at therapeutic concentrations (100 mu g/mL) inhibited DNA synthesis (10-42% decrease in [H-3]-thymidine incorporation rates) and growth (5-10% decrease in cell numbers) in HUVEC under normal cultures.Conclusion: DS, SQ, SZ and HSW are physiologically active on human vascular endothelial cells. The actions by HSW and SZ to reduce apoptosis and DS and SQ to inhibit adhesion molecule expression may help protect endothelial function and inhibit atherogenesis, while their actions to inhibit DNA synthesis and cell growth may weaken the ability of endothelial repair. Further studies are needed to identify the chemical compounds responsible to these physiological effects by these herbs. (C) 2007 Elsevier Ireland Ltd. All rights reserved.