Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding
Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding
复制标题
重新审视 delta-1-吡咯啉-5-羧酸合成酶在耐旱作物育种中的作用
DOI：
10.1016/j.cj.2022.04.002
复制
发表时间：
2022-08-01
期刊：
影响因子：
6.6
通讯作者：
Lu, Yanli
中科院分区：
文献类型：
--
作者：
Feng, Xuanjun;Hu, Yue;Lu, Yanli
δ-1-吡咯啉-5-羧酸合成酶（P5CS）基因在胁迫植物中发挥保护功能。然而，P5CS 引起的脯氨酸积累与植物非生物胁迫耐受性之间的关系并不总是清楚，因为在一些报告中已报道 P5CS 过度表达会抑制正常条件下的植物生长。我们通过在ABA诱导启动子的调控下表达AtP5CS1和反馈抑制去除的AtP5CS1 (AtP5CS1F128A)基因来重新评估P5CS在耐旱水稻育种中的作用，以避免正常条件下P5CS过度表达的潜在副作用。 ABA 诱导的 AtP5CS1 和 AtP5CS1F128A 增加了营养液中幼苗的生长（在渗透胁迫下）和盆栽植物的籽粒产量。然而，AtP5CS1对田间籽粒品质、分蘖数和产量的明显有害影响表明P5CS不适合用于耐旱育种。(c) 2022中国作物科学学会和中国农业科学院作物科学研究所。由Elsevier B.V. 代表科爱通讯有限公司制作和主持
The delta-1-pyrroline-5-carboxylate synthetase (P5CS) gene exercises a protective function in stressed plants. However, the relationship between proline accumulation caused by P5CS and abiotic stress toler-ance in plants is not always clear, as P5CS overexpression has been reported to repress plant growth under normal conditions in several reports. We re-evaluated the role of P5CS in drought-tolerant rice breeding by expressing the AtP5CS1 and feedback-inhibition-removed AtP5CS1 (AtP5CS1F128A) genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CS overexpres-sion under normal conditions. ABA-inducible AtP5CS1 and AtP5CS1F128A increased seedling growth in a nutrient solution (under osmotic stress) and grain yield in pot plants. However, the evidently deleterious effects of AtP5CS1 on grain quality, tiller number, and grain yield in the field indicated the unsuitability of P5CS for drought-tolerance breeding.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.