Analysis of recognition of fructose by imprinted polymers.
Analysis of recognition of fructose by imprinted polymers.
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DOI：
10.1016/j.talanta.2008.05.022
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发表时间：
2008-09
期刊：
影响因子：
6.1
通讯作者：
R. Rajkumar;A. Warsinke;H. Möhwald;F. Scheller;M. Katterle
中科院分区：
文献类型：
--
作者：
R. Rajkumar;A. Warsinke;H. Möhwald;F. Scheller;M. Katterle
在间歇和流动模式下研究了果糖与果糖印迹聚合物（MIP（Frc））和频哪醇印迹聚合物（对照）的结合。分析了交联剂二甲基丙烯酸乙二醇酯（EDMA）和三甲基丙烯酸异丙酯（TRIM）对粘结性能的影响。TRIM交联的MIPs显示出与EDMA-MIPs相比，对对照聚合物（频哪醇印迹的）的较低（非特异性）结合和对果糖的较高结合。此外，利用热敏电阻研究了TRIM交联分子印迹聚合物与果糖及其相应模板分子的相互作用。在0.5- 10 mM范围内实现了果糖的无标记检测。果糖与MIP的硼酸部分的特异性结合和与基质的非特异性结合之间的焓变差异导致比批结合研究高18倍的表观印迹因子。MIP传感器的交叉反应性研究表明，果糖与MIP的相互作用比二糖产生更高的信号。本文所述的研究表明，直接表征分子结合事件的潜力。
Binding of fructose to the fructose imprinted polymer (MIP(Frc)) and pinacol imprinted polymer (control) were studied both in batch and a flow through mode. The influence of the cross-linkers ethylene glycol dimethacrylate (EDMA) and trimethylolpropane trimethacrylate (TRIM) on the binding characteristics was analysed. TRIM cross-linked MIPs showed a lower (unspecific) binding for the control polymer (pinacol imprinted) and higher binding of fructose as compared with the EDMA-MIPs. Furthermore interactions of a TRIM cross-linked molecularly imprinted polymer against fructose and its corresponding template were studied using a thermistor. Label-free detection of fructose was realised in the range of 0.5–10mM. The difference in enthalpy changes between specific binding of fructose to boronic acid moieties of the MIP and non-specific binding to the matrix leads to an 18-fold higher apparent imprinting factor than batch binding studies. Cross-reactivity studies using MIP sensor indicate that the interaction of fructose to MIP generates higher signal than disaccharides. The studies described in this paper demonstrate the potential of direct characterisation of molecular binding events.