Effects of vasopressin on the isolated perfused human collecting tubule.
Effects of vasopressin on the isolated perfused human collecting tubule.
复制标题
加压素对离体灌注的人集合管的影响。
DOI：
10.1038/ki.1981.70
复制
发表时间：
1981
影响因子：
19.6
通讯作者：
Fine,LG
中科院分区：
文献类型：
--
作者：
Yanagawa,N;Trizna,W;Bar-Khayim,Y;Fine,LG
方法将手术中获得的全部或部分肾切除标本直接转移到UCLA健康科学中心的外科病理实验室，在那里获得适当的诊断标本。如果发现足够的邻近健康肾组织，则将其取出进行研究。用于研究的切片的光学显微镜检查显示正常组织学或仅极轻微的间质纤维化。将1至2 mm的皮质组织切片浸入冷冻的林格氏溶液中，并运送到微灌注实验室。在每种情况下，解剖开始在15至20毫米内从病人的肾脏组织切除。许可使用这些外科病理学标本用于研究目的是由加州大学洛杉矶分校人类受试者保护委员会。在2年的连续观察中，只有7个标本被发现是适合于正常肾单位段的研究。五个肾脏因肾细胞癌而被切除，两个肾脏因肾盂肾盏系统结石而被切除。
Methods Total or partial nephrectomy specimens obtained at operation were directly transferred in chilled Ringer's solution to the UCLA Center for the Health Sciences' surgical pathology laboratory where the appropriate diagnostic specimens were obtained. If an adequate piece of adjacent healthy renal tissue was found, it was removed for study. Light microscopy of the sections taken for study revealed normal histology or minimal interstitial fibrosis only. A 1-to 2-mm slice of cortical tissue was immersed in chilled Ringer's solution and transported to the microperfusion laboratory. In each case, dissection was commenced within 15 to 20 mm of removal of the kidney tissue from the patient.Permission to use these surgical pathology speci-mens for research purposes was granted by the UCLA Human Subject Protection Committee. In a 2-year period of continuous observation, only seven specimens were found to be appropriate for the study of normal nephron segments. Five kidneys were removed due to the presence of renal cell carcinoma and two due to calculi in the pyelocalyceal system.