Transient effects of anesthetics on dendritic spines and filopodia in the living mouse cortex.
Transient effects of anesthetics on dendritic spines and filopodia in the living mouse cortex.
复制标题
DOI：
10.1097/aln.0b013e318229a660
复制
发表时间：
2011-10
期刊：
影响因子：
8.8
通讯作者：
Gan WB
中科院分区：
文献类型：
--
作者：
Yang G;Chang PC;Bekker A;Blanck TJ;Gan WB
麻醉剂被广泛用于在手术过程中诱导无意识、疼痛缓解和不动。目前还不清楚麻醉剂的使用是否对大脑中突触发育和可塑性有显著和持久的影响。为了解决这个问题，我们研究了树突棘的形成和消除，兴奋性突触的突触后位点，在发育中的小鼠皮层期间和麻醉剂暴露后。本研究使用在第5层锥体神经元中表达黄色荧光蛋白的转基因小鼠。1月龄的小鼠在数小时内经历氯胺酮-甲苯噻嗪和异氟烷麻醉。树突棘和丝状伪足，棘的前体，消除和形成率，随后在初级体感皮层使用经颅双光子显微镜数小时至数天。4-5在每个实验条件下检查动物。使用Student t检验和Mann-Whitney U检验分析数据。氯胺酮-甲苯噻嗪或异氟烷给药迅速改变树突状丝状伪足动力学，但对脊柱动力学无显著影响。在4小时麻醉期间，氯胺酮-甲苯噻嗪增加了丝状伪足的形成，而异氟烷减少了丝状伪足的消除。这两种效应都是短暂的，在动物醒来后一天内消失。我们的研究表明，暴露于麻醉剂短暂影响树突丝状伪足的动力学，但没有显着影响树突棘的发展和可塑性在1个月大的小鼠皮层。
Anesthetics are widely used to induce unconsciousness, pain relief and immobility during surgery. It remains unclear whether the use of anesthetics has significant and long lasting effects on synapse development and plasticity in the brain. To address this question, we examined the formation and elimination of dendritic spines, postsynaptic sites of excitatory synapses, in the developing mouse cortex during and after anesthetics exposure. Transgenic mice expressing yellow fluorescence protein in layer 5 pyramidal neurons were used in this study. Mice at 1 month of age underwent ketamine-xylazine and isoflurane anesthesia over a period of hours. The elimination and formation rates of dendritic spines and filopodia, the precursors of spines, were followed over hours to days in the primary somatosensory cortex using transcranial two-photon microscopy. 4–5 animals were examined under each experimental condition. Student's t-test and Mann-Whitney U-test were used to analyze the data. Administration of either ketamine-xylazine or isoflurane rapidly altered dendritic filopodial dynamics but had no significant effects on spine dynamics. Ketamine-xylazine increased filopodial formation while isoflurane decreased filopodial elimination during 4 hours of anesthesia. Both effects were transient and disappeared within a day after the animals woke up. Our studies suggest that exposure to anesthetics transiently affects the dynamics of dendritic filopodia but has no significant effect on dendritic spine development and plasticity in the cortex of 1-month-old mice.