Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs
Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs
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DOI：
10.1016/j.brainres.2010.03.026
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发表时间：
2010-05-12
期刊：
影响因子：
2.9
通讯作者：
Haertig, Wolfgang
中科院分区：
文献类型：
--
作者：
Alpar, Alan;Kuenzle, Heinz;Haertig, Wolfgang
除了突触重塑，新神经元的形成越来越被认为是中枢神经系统可塑性变化的重要线索。尽管所有的脊椎动物都保留了适度的神经增殖能力，但在衰老过程中，遗传学上年轻的哺乳动物在这种潜力方面会受到严重损害。目前的研究表明，较小的刺猬tenrec，一个低脑化指数的食虫动物，在衰老过程中保留其神经原性潜力令人惊讶地好。这是通过定量分析5-溴-2 '-脱氧尿苷（BrdU）免疫标记的嗅球，古，阿尔奇，和新皮质从2至7岁的动物。除了这些新生的细胞，大量先前形成的未成熟神经元在整个成年期都存在，如在各种前脑区域（包括原始皮质、古皮质、杏仁核和杏仁核）中的双皮质素（DCX）免疫染色所示。在嗅球和海马中的一些脑室相关细胞被BrdU和DCX免疫反应性双重标记。然而，大多数DCX细胞在古皮质可以被认为是持久的未成熟的神经元，显然不进入分化程序，因为双荧光标记并没有揭示他们的共存与众多的神经元标记，而只有一小部分共表达泛神经元标记HuC/D。最后，本研究揭示了tenrecs作为合适的实验室动物来研究年龄依赖性脑改变（例如，神经发生）或缓慢的退化过程，特别是由于与小鼠和大鼠相比，tenrecs的寿命至少增加了一倍。(C)2010 Elsevier B. V.保留所有权利。
In addition to synaptic remodeling, formation of new neurons is increasingly acknowledged as an important cue for plastic changes in the central nervous system. Whereas all vertebrates retain a moderate neuroproliferative capacity, phylogenetically younger mammals become dramatically impaired in this potential during aging. The present study shows that the lesser hedgehog tenrec, an insectivore with a low encephalization index, preserves its neurogenic potential surprisingly well during aging. This was shown by quantitative analysis of 5-bromo-2'-deoxyuridine (BrdU) immunolabeling in the olfactory bulb, paleo-, archi-, and neocortices from 2- to 7-year-old animals. In addition to these newly born cells, a large number of previously formed immature neurons are present throughout adulthood as shown by doublecortin (DCX) immunostaining in various forebrain regions including archicortex, paleocortex, nucleus accumbens, and amygdala. Several ventricle-associated cells in olfactory bulb and hippocampus were double-labeled by BrdU and DCX immunoreactivity. However, most DCX cells in the paleocortex can be considered as persisting immature neurons that obviously do not enter a differentiation program since double fluorescence labeling does not reveal their co-occurrence with numerous neuronal markers, whereas only a small portion coexpresses the pan-neuronal marker HuC/D. Finally, the present study reveals tenrecs as suitable laboratory animals to study age-dependent brain alterations (e.g., of neurogenesis) or slow degenerative processes, particularly due to the at least doubled longevity of tenrecs in comparison to mice and rats. (C) 2010 Elsevier B.V. All rights reserved.