An unusual sexually dimorphic mosaic distribution of a subset of kallikreins in the granular convoluted tubule of the mouse submandibular gland detected by an antibody with restricted immunoreactivity.
An unusual sexually dimorphic mosaic distribution of a subset of kallikreins in the granular convoluted tubule of the mouse submandibular gland detected by an antibody with restricted immunoreactivity.
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通过免疫反应性受限的抗体检测到，小鼠颌下腺颗粒曲管中激肽释放酶亚群存在异常的两性二态性嵌合分布。
DOI：
10.1023/a:1003506302065
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hosoi,K
中科院分区：
文献类型：
--
作者：
Kurabuchi,S;Da,JT;Gresik,EW;Hosoi,K
小鼠下颌下腺颗粒曲小管含有多种生物活性蛋白，包括几种激肽释放酶。小管是在多激素调节下，是两性异形的，男性比女性大。相应地，其各种蛋白质分泌产物的水平在男性中比在女性中更丰富。然而，等电聚焦研究表明，真正的组织激肽释放酶，mK 1，是更丰富的女性比男性下颌下腺。在这项研究中，制备的抗血清与小鼠mK 1的限制性免疫反应，并可能在小鼠下颌下腺的低丰度的其他激肽释放酶家族成员，并用于免疫细胞化学染色的颗粒曲管细胞在成年雄性和雌性小鼠的下颌下腺，通过间接酶标记和免疫金标记的抗体方法，分别进行光镜和电镜。免疫反应阳性的小管细胞的分布显示出不寻常的性二型性。在雄性中，仅少数分散的细长小管细胞被强染色，而更典型的大小管细胞仅偶尔呈弱阳性，其中许多细胞未染色。相比之下，在雌性中未观察到细长的小管细胞，并且约三分之二的更典型的小管细胞显示出中度至强烈的免疫染色。免疫电镜显示，免疫染色仅限于颗粒曲小管细胞的分泌颗粒在两种性别。雄性的细长小管细胞有许多强染色的小顶端分泌颗粒和偶尔的基底内折叠;在弱阳性的较大的更典型的小管细胞中，并非所有分泌颗粒都是阳性的，阳性颗粒的染色强度存在颗粒间差异。在雌性动物中，尽管染色的小管细胞较多，但也观察到染色强度的粒间变化。在两种性别中，许多小管细胞不含任何分泌颗粒，显示免疫金标记的激肽释放酶。这些发现证实，与大多数颗粒曲小管蛋白的情况相反，mK 1和可能的其他次要激肽释放酶家族成员在雌性小鼠的颗粒曲小管中更丰富，并且这些激肽释放酶的含量不仅在不同的小管细胞之间存在相当大的差异，而且在任何性别的任何给定小管细胞中的单个分泌颗粒中也存在相当大的差异。
The granular convoluted tubule of the mouse submandibular gland contains a wide variety of biologically active proteins, including several kallikreins. The tubule is under multihormonal regulation, and is sexually dimorphic, being larger in males than in females. Correspondingly, levels of its various protein secretory products are more abundant in males than in females. However, isoelectric focussing studies show that the true tissue kallikrein, mK1, is more abundant in the female than in the male submandibular gland. In this study, an antiserum was prepared with restricted immunoreactivity for mouse mK1, and possibly other kallikrein family members of low abundance in the mouse submandibular gland, and used for the immunocytochemical staining of the granular convoluted tubule cells in the submandibular gland of adult male and female mice, by indirect enzyme-labeled and immunogold-labeled antibody methods for light and electron microscopy, respectively. The distribution of immunoreactive tubule cells showed an unusual sexual dimorphism. In males only a few scattered slender tubule cells were strongly stained, while the more typical large tubule cells were only occasionally weakly positive, and many of them were not stained. By contrast, in females slender tubule cells were not seen, and about two thirds of the more typical tubule cells showed moderate to strong immunostaining. Immunoelectron microscopy revealed that immunostaining was confined to the secretion granules in granular convoluted tubule cells in both sexes. The slender tubule cells of males had many strongly stained small apical secretion granules and occasional basal infoldings; in the weakly positive larger more typical tubule cells not all secretion granules were positive, and there was intergranular variation in the intensity of staining of positive granules. In females, although more tubule cells were stained, intergranular variations in staining intensity were also noted. In both sexes, many tubule cells did not contain any secretion granules that showed immunogold labeling for kallikreins. These findings establish that, in contrast to the situation for the majority of granular convoluted tubules proteins, mK1 and possibly other minor kallikrein family members are more abundant in the granular convoluted tubules of female mice, and that there is considerable variation in the content of these kallikreins not only between different tubule cells, but also in individual secretion granules in any given tubule cell in either sex.