Cross-sectional study of biomarkers of exposure and biological effect on monozygotic twins discordant for smoking
Cross-sectional study of biomarkers of exposure and biological effect on monozygotic twins discordant for smoking
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DOI：
10.1515/cclm.2011.009
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发表时间：
2011-01-01
影响因子：
6.8
通讯作者：
Bassi, Antonella
中科院分区：
文献类型：
--
作者：
Andreoli, Cristina;Gregg, Evan O.;Bassi, Antonella
背景资料：本研究的目的是调查吸烟状态和暴露的生物标志物（BoE）和生物效应（BoBE）在吸烟状态不一致的单卵双胞胎（吸烟者和非吸烟者对）之间的可能相关性。通过消除潜在的遗传变异，以这种方式，一个更清晰的模式的生活方式和环境暴露的影响应该变得apparent.Methods：这是一个横截面研究单卵健康双胞胎（44名受试者，26名男性和18名女性），平均年龄31.5岁。BoE吸烟和BoBE测量体液（24小时尿液和血液）后，医学pre-screening.Results：所有BoE吸烟者双胞胎显着较高。在BoBE中，与非吸烟双胞胎相比，吸烟双胞胎的11-脱氢血栓烷B-2（11-脱氢TBX）、2，3-二去甲血栓烷B-2（2，3-二去甲TBX）、8-表-前列腺素F2 α（8-epiPGF）、羟脯氨酸（OH-P）、纤维蛋白原、白色血细胞（WBC）、中性粒细胞和淋巴细胞计数以及心率在统计学上显著增加。此外，中性粒细胞计数与11-TBX（r = 0.32）、WBC与8-epiPGF（r = 0.33）、OH-P与8-epiPGF（r = 0.49）、心率与纤维蛋白原（r = 0.46）之间存在显著相关性。此外，BoBE的一个子集，被报道为与炎症性疾病和血管疾病的早期阶段，已经出现，显示出与吸烟状态从本研究和以前的研究一致的关系。通过使用同卵双生子对，遗传变异性已被排除作为本研究中变异性的可能来源。这些结果应有助于解释使用这些生物标志物的其他人群研究。
Background: The aim of this study was to investigate the possible correlation between smoking status and biomarkers of exposure (BoE) and biological effect (BoBE) in monozygotic twins discordant for smoking status (smoker and non-smoker pairs). By eliminating potential genetic variability in this manner, a clearer pattern of the effects of lifestyle and environmental exposures should become apparent.Methods: This was a cross-sectional study on monozygotic healthy twins (44 subjects, 26 males and 18 females) with a mean age 31.5 years. BoE to cigarette smoke and BoBE were measured in body fluids (24 h urine and blood) after medical pre-screening.Results: All BoE were significantly higher in the smoker twins. Among BoBE, 11-dehydrothromboxane B-2 (11-dehydro TBX), 2,3-dinorthromboxane B-2 (2,3-dinor TBX), 8-epi-prostaglandin F2 alpha (8-epiPGF), hydroxyproline (OH-P), fibrinogen, white blood cell (WBC), neutrophil and lymphocyte counts and heart rate were statistically significantly increased in the smoker compared to the non-smoker twins. Moreover, statistically significant correlations between neutrophil count and 11-dehydro TBX (r = 0.32), WBC and 8-epiPGF (r = 0.33), OH-P and 8-epiPGF (r = 0.49) and heart rate and fibrinogen (r = 0.46) were observed.Conclusions: The study results confirmed the reliability of the BoE for the evaluation of smoking status. Moreover, a subset of the BoBE, reported as being associated with inflammatory conditions and early stages of vascular disorders, has emerged as showing a consistent relationship with smoking status from the present and the previous studies. By using monozygotic twin pairs, genetic variability has been excluded as a possible source of variability in this study. These results should assist in the interpretation of other population studies using these biomarkers.