Properties and proteolysis of ferric enterobactin outer membrane receptor in Escherichia coli K12.
Properties and proteolysis of ferric enterobactin outer membrane receptor in Escherichia coli K12.
复制标题
大肠杆菌 K12 中铁肠杆菌素外膜受体的特性和蛋白水解。
DOI：
10.1021/bi00261a050
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Neilands,JB
中科院分区：
文献类型：
--
作者：
Fiss,EH;Stanley-Samuelson,P;Neilands,JB
材料与方法材料。0.5Mhc1中的55FeCl3从ICN公司购买，DEAE-纤维素以DE-52的形式从Whatman公司购买。肠杆菌素是从大肠杆菌AN102(Wayne et al.，1976)中分离得到的，[55Fe]亚铁按邻苯二酚铁的形成过程制备，粘菌素B由Hollifield&Neilands(1978)提供的方法得到。双(2，3-二羟基苯甲酰基)赖氨酸是由T.Peterson和A.Chimiak在本实验室通过改进Corbin&Bulen(1969)的方法合成的。生物胶A5M和A5M由Bio-Rad实验室提供。细菌菌株。来自大肠杆菌K12的细菌菌株包括BN3040和UT4400，以前已经描述过(Fiss等人，1979)。质粒DNA pGGCllO是pMC44(Gayda&Markovitz，1978)的pBR322衍生物，而大肠杆菌B、大肠杆菌B/r和鼠伤寒沙门氏菌LT2来自实验室。
Materials and Methods Materials. 55FeCl3 in 0.5 Mhc1 was purchased from ICN Corp. DEAE-cellulose was purchased from Whatman Corp. in the form of DE-52. Enterobactin was isolated from E. coli strain AN102 as previously described (Wayne et al., 1976).[55Fe] Ferric enterobactin, prepared according to the procedure for formation of ferric catecholates, and colicin B were obtained by the methods given by Hollifield & Neilands (1978). Bis (2, 3-dihydroxybenzoyl) lysine was synthesized in this lab-oratory by T. Peterson and A. Chimiak via a modification of the method of Corbin & Bulen (1969). Bio-Gels A5M and A. 5M were obtained from Bio-Rad Laboratories. Bacterial Strains. Bacterial strains derived from E. coli K12 include BN3040 and UT4400 andhave been previously described (Fiss et al., 1979). Plasmid DNA pGGCllO is a pBR322 derivative of pMC44 (Gayda & Markovitz, 1978), while E. coli B, E. coli B/r and Salmonella typhimurium LT2 are from laboratory stocks.