Amniotic lamellar body count and congenital diaphragmatic hernia in humans and in a rat model.
Amniotic lamellar body count and congenital diaphragmatic hernia in humans and in a rat model.
复制标题
人类和大鼠模型中的羊膜板层计数和先天性膈疝。
作者：
Watanabe Y;Tsuda H;Kotani T;Sumigama S;Mano Y;Hayakawa M;et al.
背景：我们使用羊膜板层体计数（LBC）检测先天性膈疝（CDH）胎儿肺发育不良和肺成熟的程度。我们评估了LBC、磁共振成像（MRI）和超声检查结果对CDH预后的预测。我们在胚胎第21天（E）从正常和除草醚诱导的CDH大鼠（在E9口服100 mg）收集新生儿羊水和肺组织。结果：CDH存活组LBC明显高于死亡组（P< 0.01）; LBC与MRI上胎儿肺体积百分比呈显著正相关（P< 0.001; r= 0.716）。在大鼠中，LBC是显着高于在CDH大鼠（P< 0.01），并与胎肺weight.Conclusion相关：我们得出结论，LBC是有用的预测肺发育不良的人CDH后35孕周和在一个大鼠模型的除草醚诱导的CDH。
Background:We examined the extent of fetal lung hypoplasia and lung maturation using the amniotic lamellar body count (LBC) in congenital diaphragmatic hernia (CDH).Methods:We obtained 30 amniotic fluid samples from human CDH cases during cesarean section. We assessed LBC, magnetic resonance imaging (MRI), and ultrasound findings for predicting the prognosis of CDH. We collected newborn amniotic fluid and lung tissue at embryonic day (E) 21 from normal and nitrofen-induced CDH rats (administered 100 mg orally at E9). Amniotic LBCs in rats were measured using light microscopy.Results:In human CDH, LBC was significantly higher in the surviving than in the deceased group (P< 0.01). A significant positive correlation was observed between LBC and percentage of fetal lung volume on MRI (P< 0.001; r= 0.716). In rats, LBC was significantly higher in controls than in CDH rats (P< 0.01) and correlated with fetal lung weight.Conclusion:We conclude that LBC is useful for predicting lung hypoplasia in human CDH after 35 gestational weeks and in a rat model of nitrofen-induced CDH.