Response of the adult rat to orchidectomy and ovariectomy as determined by LH radioimmunoassay.
Response of the adult rat to orchidectomy and ovariectomy as determined by LH radioimmunoassay.
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通过 LH 放射免疫测定确定成年大鼠对睾丸切除术和卵巢切除术的反应。
DOI：
10.1210/endo-84-6-1359
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发表时间：
1969
期刊：
影响因子：
4.8
通讯作者：
A. Midgley
中科院分区：
文献类型：
--
作者：
Vernon L. Gay;A. Midgley
将雄性和雌性大鼠（成年时去势）通过心脏穿刺反复流血的或通过腹主动脉放血。在睾丸切除后8小时内（但不是在1、2、3或5小时），用放射免疫测定法测定的血清LH浓度显著（p <0.01）并持续增加，在24小时内，血清LH浓度比正常雄性对照组高50倍。虽然平均血清LH浓度在睾丸切除后第2天至第61天增加约2倍，但随后的增量从未接近第一天观察到的增加率。相比之下，卵巢切除术导致血清LH浓度逐渐增加，直到卵巢切除术后近一周才观察到与卵巢切除术后24小时相当的水平。与雌性大鼠相比，雄性大鼠对去势的反应是血清LH增加更快，平均血清LH浓度更高。虽然平均血清LH浓度...
Male and female rats, castrated as adults, were bled repeatedly by cardiac puncture or exsanguinated via the abdominal aorta. Within 8 hr (but not at 1, 2, 3 or 5 hr) after orchidectomy, serum LH concentrations, as measured by radioimmunoassay, were significantly (p <.01) and consistently increased, and within 24 hr the serum LH concentrations were as much as 50 times greater than those observed in intact male controls. Although the average serum LH concentration increased about 2-fold between the 2nd and 61st days following orchi-dectomy, subsequent increments never approached the rate of increase observed during the first day. By contrast, ovariectomy resulted in a gradual increase in serum LH concentrations; levels comparable to those seen 24 hr after orchidectomy were not observed until nearly a week after ovariectomy. When compared with female rats, male rats responded to castration with a more rapid increase in serum LH and with higher mean serum LH concentrations. Although the average serum LH conc...