Mobile and immobile adhesion of staphylococcal strains to hydrophilic and hydrophobic surfaces
Mobile and immobile adhesion of staphylococcal strains to hydrophilic and hydrophobic surfaces
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DOI：
10.1016/j.jcis.2008.11.025
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发表时间：
2009-03-01
影响因子：
9.9
通讯作者：
Busscher, Henk J.
中科院分区：
文献类型：
--
作者：
Boks, Niels P.;Kaper, Hans J.;Busscher, Henk J.
表皮葡萄球菌以相似的数量粘附到亲水性玻璃和疏水性二甲基二氯硅烷（DDS）涂覆的玻璃上，但粘附模式不同。在15 s（-1）剪切速率下实时观察葡萄球菌粘附，发现两种基质上的粘附动力学不同。吸附和解吸的事件，以实现类似数量的粘附细菌的数量是两倍以上的亲水性比疏水性DDS涂覆的玻璃。而且。玻璃上所有葡萄球菌中有22%在粘附于固定部位之前滑过表面（“移动的粘附模式”），但在DDS涂覆的玻璃上几乎不存在移动的粘附（1%）。在亲水性玻璃上，约20%的脱附事件发生在滑动脱附之前，而在疏水性DDS涂层玻璃上，2%的所有葡萄球菌直接从其粘附部位脱附。由于葡萄球菌和疏水性DDS涂层之间的酸碱相互作用是有吸引力的，因此建议这些相互作用促进细菌更接近，从而增强局部高亲和力位点的固定粘附。或者，如果局部位点亲和力低，这可能会导致脱附。在缺乏有吸引力的酸碱相互作用的情况下，如在亲水性玻璃上，细菌可以在DLVO-相互作用能曲线的最小值中被捕获，但这并不能阻止它们在流动下在离基质表面固定距离处滑动，直到分别在局部高或低亲和力位点处固定或解吸。(C)2008年爱思唯尔公司All rights reserved.
Staphylococcus epidermidis adheres to hydrophilic glass and hydrophobic dimethyldichlorosilane (DDS)-coated glass in similar numbers, but in different modes. Real-time observation of staphylococcal adhesion under a shear rate of 15 s(-1) revealed different adhesion dynamics on both substrata. The number of adsorption and desorption events to achieve a similar number of adhering bacteria was twofold higher on hydrophilic than on hydrophobic DDS-coated glass. Moreover. 22% of all staphylococci on glass slid over the surface prior to adhering on a fixed site ("mobile adhesion mode"), but mobile adhesion was virtually absent (1%) on DDS-coated glass. Sliding preceded desorption on hydrophilic glass in about 20% of all desorption events, while on hydrophobic DDS-coated glass 2% of all staphylococci desorbed straight from their adhesion site. Since acid-base interactions between the staphylococci and a hydrophobic DDS-coating are attractive, it is suggested that these interactions facilitate a closer approach of the bacteria and therewith enhance immobile adhesion at local, high affinity sites. Alternatively, if the local site is low affinity, this may lead to desorption. In the absence of attractive acid-base interactions, as on hydrophilic glass, bacteria can be captured in the minimum of the DLVO-interaction energy curve, but this does not prevent them from sliding under flow at a fixed distance from a substratum surface until immobilization or desorption at or from a local high or low affinity site, respectively. (C) 2008 Elsevier Inc. All rights reserved.