CCL5 and related genes might be the potential diagnostic biomarkers for the therapeutic strategies of rheumatoid arthritis
CCL5 and related genes might be the potential diagnostic biomarkers for the therapeutic strategies of rheumatoid arthritis
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DOI：
10.1007/s10067-019-04533-1
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发表时间：
2019-04
影响因子：
3.4
通讯作者：
Yinger Huang;Songyuan Zheng;Ran Wang;Cuiping Tang;Junqing Zhu;Juan Li
中科院分区：
文献类型：
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作者：
Yinger Huang;Songyuan Zheng;Ran Wang;Cuiping Tang;Junqing Zhu;Juan Li
风湿性关节炎（RA）是风湿性疾病中的常见病。方法从GEO数据库中下载GSE 1919、GSE 55235、GSE 55457和GSE 77928的基因表达谱，分析其与RA发病的关系。上述四个系列共76个样本，包括44例RA患者和32例正常对照。结果表达上调的DEGs在免疫应答、免疫系统过程的正向调节和免疫系统过程的调节等生物学过程中显著富集，而下调的DEGs在生物学过程中显著富集，包括对含氧化合物的反应、细胞脂质代谢过程和脂质代谢过程。KEGG通路分析显示，上调的DEG富集于精氨酸-细胞因子受体相互作用、趋化因子信号通路和原发性免疫缺陷。从PPI网络中识别出104个在至少两个数据集中在患者和正常对照之间表达显著差异的枢纽基因，子网络揭示这些基因参与重要的通路，包括精氨酸-细胞因子受体相互作用，趋化因子信号通路，结论本研究表明，DEG和中枢基因的鉴定促进了我们对原发性免疫缺陷的分子机制的理解。在RA的发展中，C-C基序趋化因子5（CCL 5）可能具有负面影响。CCL 5及其相关基因可能成为RA诊断和治疗的潜在生物标志物。
ObjectiveRheumatoid arthritis (RA) is a common disease of rheumatic diseases. The aim of this study was to identify gene signatures in RA and uncover their potential mechanisms.MethodGene expression profiles of GSE1919, GSE55235, GSE55457, and GSE77928 were downloaded from GEO database. The above four series contained 76 samples, including 44 RA patients and 32 normal controls. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, and protein–protein interaction (PPI) network of the differentially expressed genes (DEGs) was constructed by Cytoscape software.ResultsUp-regulated DEGs were significantly enriched in biological processes, including immune response, positive regulation of immune system process and regulation of immune system process, while down-regulated DEGs were significantly enriched in biological processes, including response to oxygen-containing compound, cellular lipid metabolic process, and lipid metabolic process. KEGG pathway analysis showed the up-regulated DEGs were enriched in cytokine–cytokine receptor interaction, chemokine signaling pathway, and primary immunodeficiency. The 104 hub genes, which were significantly differently expressed between patients and normal controls in at least two datasets, were identified from the PPI network, and subnetworks revealed that these genes were involved in significant pathways, including cytokine–cytokine receptor interaction, chemokine signaling pathway, and primary immunodeficiency.ConclusionThe present study indicated that the identified DEGs and hub genes promote our understanding of molecular mechanisms underlying the development of RA, such as C-C motif chemokine 5 (CCL5), might have a negative impact in the development of RA. CCL5 and its related genes might be the potential diagnostic biomarkers for the therapeutic strategies of RA.