SPIRAL DEFECTS IN MOTILITY ASSAYS - A MEASURE OF MOTOR PROTEIN FORCE
SPIRAL DEFECTS IN MOTILITY ASSAYS - A MEASURE OF MOTOR PROTEIN FORCE
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DOI：
10.1103/physrevlett.75.176
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发表时间：
1995-07-03
影响因子：
8.6
通讯作者：
LIBCHABER, A
中科院分区：
文献类型：
--
作者：
BOURDIEU, L;DUKE, T;LIBCHABER, A
在常用的运动性测定中，观察到细胞骨架丝在被马达蛋白包被的表面上滑动。运动中的缺陷常常会中断纤维的流动。对这样一种缺陷的检查，其中一个细丝采用螺旋形式并围绕固定点旋转，提供了一个简单的测量马达蛋白所施加的力的方法。我们证明了这种方法的普遍性，通过估计肌球蛋白和驱动蛋白的基本力量。
In a commonly used motility assay, cytoskeletal filaments are observed as they glide over a surface coated with motor proteins. Defects in the motion frequently interrupt the flow of filaments. Examination of one such defect, in which a filament adopts a spiral form and rotates about a fixed point, provides a simple measure of the force exerted by the motor proteins. We demonstrate the universality of this approach by estimating the elementary forces of both myosin and kinesin.