BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE).
BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE).
复制标题
水疱性口炎病毒（印第安纳血清型）的两个斑块变种的生物特性。
作者：
R. Wagner;Allan H. Levy;R. M. Snyder;Gilbert A. Ratcliff;David F. Hyatt
从水疱性口炎（VS）病毒印第安那血清型圣胡安株中克隆出大菌斑（LP）和小菌斑（SP）变异株。这两种变异在鸡胚（CE）和L细胞培养中均能繁殖。当在L细胞培养中比较两种变体时，感染性、细胞致病性和生长潜力的差异更为明显。LP和SP变体在以下特性上似乎没有显着差异：在CE或L细胞上的吸附，从感染的L细胞中释放，热稳定性，在琼脂提取物存在下的生长，斑块大小和在碳酸氢盐浓度降低的条件下的生长，紫外线照射和细胞毒性的传染性失活。在氯化铯密度梯度中，可以检测到抗体中和和浮力密度的轻微变化。
Large plaque (LP) and small plaque (SP) variants were cloned from stock cultures of Indiana serotype, San Juan strain of vesicular stomatitis (VS) virus. Both variants bred true in cultures of chick embryo (CE) and L cells. Differences in infectivity, cytopathogenicity and growth potential were more pronounced when the two variants were compared in cultures of L cells. The LP and SP variants did not appear to differ significantly in the following properties: adsorption on CE or L cells, release from infected L cells, thermostability, growth in the presence of agar extracts, plaque size and growth under conditions of reduced bicarbonate concentrations, inactivation of infectivity by ultraviolet irradiation and cytotoxicity. Slight variations could be detected in neutralization by antibody and buoyant density in a cesium chloride density gradient.
The LP variant was not conclusively inferior in its capacity to induce formation of interferon during the course of infection. However, definite evidence was obtained that the SP variant is more susceptible to the action of an exogenous interferon produced by infected L cells.
Persistent infection of L cells could be established only with the SP variant. During an observation period of 5 months, carrier L cell cultures underwent periodic cycles of SP virus multiplication and cell destruction alternating with cycles of virus quiescence and slow repopulation of cultures by surviving L cells. No interferon could be detected in SP carrier cultures, but the L cells became more resistant to superinfection with the LP variant.