A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area
A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area
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DOI：
10.1002/ps.5643
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发表时间：
2019-11-17
影响因子：
4.1
通讯作者：
Calvitti, Maurizio
中科院分区：
文献类型：
--
作者：
Caputo, Beniamino;Moretti, Riccardo;Calvitti, Maurizio
背景需要新的工具来减少与白纹伊蚊在温带地区的殖民有关的外来虫媒病毒传播的滋扰和风险。不亲和昆虫技术(IIT)是一种基于雄性与被操纵的内共生体和野生雌性之间细胞质不亲和性的种群抑制方法。在这里，我们介绍了欧洲第一个评估Ae能力的野外实验的结果。白纹伊蚊(Arbopictus line，ARwP)去除其天然内共生沃尔巴克氏菌，并用蚊子库蚊的沃尔巴克氏菌株感染，以使野生雌性绝育。结果我们在意大利罗马市区的绿化区每周放生约4500只雄性ARWP，连续6周，并进行了卵子(N=13442)、雌性(N=128)和雄性(N=352)的采集。卵子(N=13783)和雌性(N=48)的采集也在两个未经处理的对照地点进行。在释放过程中，平均而言，处理地点的可存活卵子百分比显著低于对照地点，其中以第四次释放后的差异最大(16%)。在第一次释放雄性ARwP后第3天到最后一次释放后第7天，释放点的ARwP与野生雄性的比例平均为7：10，释放的雄性存活时间长达2周。与对照地点相比，在放行地点采集的大约30%的雌性蚊子表现出100%的不育性，20%的雌性蚊子表现出严重的育性降低。结论结果支持IIT作为促进Ae的工具的潜力。在城市背景下控制白纹伊蚊，并强调有必要进行更大规模的实地试验，以评估该方法在抑制野生种群方面的成本效益。(三)2019年化学工业学会
BACKGROUND Novel tools are needed to reduce the nuisance and risk of exotic arbovirus transmission associated with the colonization of temperate regions by Aedes albopictus. The incompatible insect technique (IIT) is a population suppression approach based on cytoplasmic incompatibility between males with manipulated endosymbionts and wild females. Here, we present the results of the first field experiment in Europe to assess the capacity of an Ae. albopictus line (ARwP) deprived of its natural endosymbiont Wolbachia and transinfected with a Wolbachia strain from the mosquito Culex pipiens, to sterilize wild females. RESULTS We released similar to 4500 ARwP males weekly for 6 weeks in a green area within urban Rome (Italy) and carried out egg (N = 13 442), female (N = 128) and male (N = 352) collections. Egg (N = 13 783) and female (N = 48) collections were also carried out at two untreated control sites. The percentage of viable eggs during release was, on average, significantly lower in treated sites than in control sites, with the greatest difference (16%) seen after the fourth release. The ARwP to wild male ratio in the release spots between day 3 after the first ARwP male release and day 7 after the last release was, on average, 7:10. Released males survived up to 2 weeks. Approximately 30% of females collected in the release spots showed 100% sterility and 20% showed strongly reduced fertility compared with control sites. CONCLUSIONS Results support the potential of IIT as a tool contributing to Ae. albopictus control in the urban context, and stress the need for larger field trials to evaluate the cost-efficacy of the approach in suppressing wild populations. (c) 2019 Society of Chemical Industry