Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation.
Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation.
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DOI：
10.1007/978-1-4939-1453-1_19
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Schulz TJ
中科院分区：
文献类型：
--
作者：
Lau AM;Tseng YH;Schulz TJ
骨骼肌包含几种类型的细胞，其中致力于成脂血统的祖细胞群体最近才被鉴定出来。白色和棕色脂肪细胞的潜在来源，后者代表着治疗肥胖症的潜在靶点，这一领域引起了相当大的兴趣。荧光激活细胞分选(FACS)为严格定义的细胞群体的前瞻性分离提供了一种巧妙的策略。在这里，我们描述了一种流式细胞术的方法来分离具有默认潜能的肌肉成脂前体细胞进行白色成脂。我们进一步描述了一种在暴露于骨形态发生蛋白7(BMP7)后诱导向棕色样脂肪细胞系承诺的方法。
Skeletal muscle harbors several types of cells, among which a population of progenitors committed to the adipogenic lineage has only recently been identified. Potential sources of white and brown adipocytes, the latter representing a potential target to treat obesity, are of considerable interest to the field. Fluorescence-activated cell sorting (FACS) provides an elegant strategy for prospective isolation of closely defined cell populations. Here we describe a flow cytometric method to isolate muscle-resident adipogenic progenitor cells with a default potential to undergo white adipogenesis. We further describe an approach to induce commitment to a lineage of brown-like adipocytes upon exposure to bone morphogenetic protein 7 (BMP7).