Effect of chitosan solution on the inhibition of Acidovorax citrulli causing bacterial fruit blotch of watermelon.
Effect of chitosan solution on the inhibition of Acidovorax citrulli causing bacterial fruit blotch of watermelon.
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DOI：
10.1002/jsfa.5812
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发表时间：
2013-03
影响因子：
4.1
通讯作者：
Bin Li;Yu Shi;Changlin Shan;Qing Zhou;Mansoor Ibrahim;Yanli Wang;Guo-xing Wu;Hongye Li;Guan-lin Xie;Guo-chang Sun
中科院分区：
文献类型：
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作者：
Bin Li;Yu Shi;Changlin Shan;Qing Zhou;Mansoor Ibrahim;Yanli Wang;Guo-xing Wu;Hongye Li;Guan-lin Xie;Guo-chang Sun
背景我国西瓜生产受到果斑病的严重影响，目前采取的防治措施有限。壳聚糖已被用于防治多种植物病害，被认为是最有前途的防治植物病害的生化制剂。结果壳聚糖对西瓜细菌性果斑病的体外抑菌效果及对西瓜幼苗的保护作用。结果表明，3种壳聚糖，尤其是壳聚糖A在0.40 mg/mL浓度下，对瓜类食酸菌的生长有明显的抑制作用。壳聚糖A的抑菌活性受壳聚糖浓度和孵育时间的影响。透射电镜观察表明壳聚糖的直接抑菌作用可能是由于膜溶解所致。与对照相比，0.40 mg/mL壳聚糖A处理可显著降低土栽西瓜幼苗的病情指数和土栽西瓜幼苗的死亡率。壳聚糖种子处理能增加西瓜幼苗的鲜重和干重，而壳聚糖叶面喷施对西瓜幼苗的鲜重和干重没有影响。结论壳聚糖溶液对西瓜细菌性果斑病有一定的防治作用。
BACKGROUND The production of watermelon in China has been seriously hampered by fruit blotch disease and limited control measures are now applied. Chitosan has been employed to control a variety of plant diseases and is considered to be the most promising biochemical to control this disease. RESULTS The in vitro antibacterial effect of chitosan and its ability in protection of watermelon seedlings from bacterial fruit blotch were evaluated. Results showed that three types of chitosan, in particular, chitosan A at 0.40 mg mL⁻¹ significantly inhibited the growth of Acidovorax citrulli. The antibacterial activity of chitosan A was affected by chitosan concentration and incubation time. The direct antibacterial activity of chitosan may be attributed to membrane lysis evidenced by transmission electron microscopic observation. The disease index of watermelon seedlings planted in soil and the death rate of seedlings planted in perlite were significantly reduced by chitosan A at 0.40 mg mL⁻¹ compared to the pathogen control. Fresh and dry weight of watermelon seedlings planted in soil was increased by chitosan seed treatment, but not by chitosan leaf spraying. CONCLUSION The results indicated that chitosan solution may have a potential in controlling bacterial fruit blotch of watermelon.