CHANGES IN ABUNDANCE OR STRUCTURE OF THE PER GENE-PRODUCT CAN ALTER PERIODICITY OF THE DROSOPHILA CLOCK
CHANGES IN ABUNDANCE OR STRUCTURE OF THE PER GENE-PRODUCT CAN ALTER PERIODICITY OF THE DROSOPHILA CLOCK
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DOI：
10.1038/326390a0
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发表时间：
1987-03-26
期刊：
影响因子：
64.8
通讯作者：
YOUNG, MW
中科院分区：
文献类型：
--
作者：
BAYLIES, MK;BARGIELLO, TA;YOUNG, MW
控制果蝇生物节律的周期（per）基因座最初是由三种化学诱导突变定义的1。携带per °突变的果蝇是昼夜节律性的，而per和per l突变体的昼夜行为节律分别为19小时和29小时周期1。野生苍蝇有24小时的节律。在这里，我们比较了三个突变体与亲本野生型的每个位点的DNA序列。pers和perl突变导致氨基酸取代，而per °引入了早期翻译终止（琥珀）。结果表明，蛋白质产物的过控制的生物节律。我们还报告说，这种蛋白质的丰富可能会设定果蝇的步伐。虽然当果蝇被每个基因座的DNA转化时，昼夜节律恢复了，但接受相同转化DNA片段的果蝇可以产生周期相差超过12小时的节律。转录研究揭示了转化株系中perRNA水平的十倍变化。perRNA水平与周期长度呈负相关，因此，perRNA水平最低的果蝇生物钟运行缓慢。综合以上研究结果，我们认为pers和pers突变体分别产生低活性和高活性的perprotein。
Theperiod(per) locus, which controls biological rhythms inDrosophila, was originally defined by three chemically induced mutations1. Flies carrying theper°mutation were arrhythmic, whereaspersandperlmutants had circadian behavioural rhythms with 19-hour and 29-hour periodicities, respectively1. Wild-type flies have 24-hour rhythms. Here we compare theperlocus DNA sequences of the three mutants with the parental wild-type. Thepersandperlmutations lead to amino-acid substitutions, whereasper°introduces an early translation stop (amber). The results indicate that the protein product ofpercontrols biological rhythms. We also report that the abundance of this protein may set the pace of theDrosophilaclock. Although circadian rhythms are restored when arrhythmic (per–)Drosophilaare transformed withperlocus DNA, flies receiving identical transforming DNA segments can produce rhythms with periods that differ by more than 12 hours. Transcription studies reveal a tenfold variation in the level ofperRNA among transformed lines. Levels ofperRNA are inversely correlated with period length, so that flies with lowest levels of theperproduct have slow-running biological clocks. On the basis of the combined studies we suggest thatperlandpersmutants produce hypoactive and hyperactiveperproteins, respectively.