A model to study total hepatic ischemia-reperfusion injury
A model to study total hepatic ischemia-reperfusion injury
复制标题
DOI：
10.1016/j.transproceed.2004.10.031
复制
发表时间：
2004-11-01
影响因子：
0.9
通讯作者：
Davidson, BR
中科院分区：
文献类型：
--
作者：
Kanoria, S;Glantzounis, G;Davidson, BR
背景。大多数用于研究肝缺血再灌注损伤（IRI）的实验动物模型涉及肝脏部分或部分缺血或门静脉分流术以避免肠系膜充血。然而，这些并不能反映肝移植过程中发生的全局缺血。建立无门静脉分流的兔全肝缺血模型。选取雄性新西兰大白兔20只，体重3.5±0.3 kg，随机分为4组：1组（n = 5），假手术；2组（n = 5）， 20分钟全肝缺血；3组（n = 5）， 25分钟全肝缺血；第4组（n = 5）， 30min肝缺血。用非创伤性血管袢阻塞门静脉和动脉，诱导全肝缺血，再灌注2小时后测量。由于代谢性酸中毒，所有5只动物全肝缺血30分钟，在再灌注2小时造成严重肝损伤，导致心脏骤停。20分钟的全缺血是可以忍受的，没有产生明显的肝损伤。与假手术组（血清ALT， 25.4 +/- 2.7；血清AST， 47.4 +/- 3.0；血清LDH， 307.6 +/- 44.4 U/L; P < 0.003）相比，25分钟全缺血可耐受，但2小时再灌注时肝损伤显著（68 +/- 41,283.0 +/- 20.5和835.2 +/- 52.7 U/L）。家兔可以耐受25分钟的全肝缺血而不需要门静脉分流。这个25分钟的缺血模型模拟了肝移植过程中的手术条件，将在调节IRI的研究中有价值。
Background. Most experimental animal models for studying hepatic ischemia-reperfusion injury (IRI) involve partial or segmental ischemia of the liver or a portocaval shunt procedure to avoid mesenteric congestion. However, these do not reflect the global ischemia that occurs during liver transplantation. A rabbit model of total hepatic ischemia without a portocaval shunt is described.Methods. Twenty male New Zealand white rabbits (3.5 +/- 0.3 kg) were allocated to four groups: group 1 (n = 5), sham-operated; group 2 (n = 5), 20-minute total hepatic ischemia; group 3 (n = 5), 25-minute total hepatic ischemia; and group 4 (n = 5), 30-minute total hepatic ischemia. Total hepatic ischemia was induced by occluding the portal inflow vessels (portal vein and artery) with an atraumatic vascular loop and were measurements taken for 2 hours during reperfusion.Results. A total hepatic ischemia of 30 minutes caused severe liver injury resulting in cardiac arrest at 2 hours of reperfusion in all five animals due to metabolic acidosis. Twenty minutes of total ischemia was tolerated and did not produce significant liver injury. Twenty-five minutes of total ischemia was tolerated but at 2 hours of reperfusion, resulted in significant liver injury (68 +/- 41, 283.0 +/- 20.5, and 835.2 +/- 52.7 U/L) compared with the sham-operated group (serum ALT, 25.4 +/- 2.7; serum AST, 47.4 +/- 3.0; serum LDH, 307.6 +/- 44.4 U/L; P < .003).Conclusions. Rabbits can tolerate 25 minutes of total hepatic ischemia without a portosystemic shunt. This 25-minute ischemia model simulates operative conditions during liver transplantation and will be valuable in studies modulating IRI.