The effect of incomplete fixation of elastin on the appearance of pulmonary alveoli.
The effect of incomplete fixation of elastin on the appearance of pulmonary alveoli.
复制标题
弹性蛋白不完全固定对肺泡外观的影响。
DOI：
10.1115/1.3138307
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Tremer,HM
中科院分区：
文献类型：
--
作者：
Sobin,SS;Fung,YC;Tremer,HM
通过快速冷冻肺、用冰冷冻替代、用四氧化锇在非水和非极性溶剂中固定干燥肺以及火棉胶包埋制备的组织切片中的肺泡几何形状与通过在气道中滴注水性醛或四氧化锇溶液制备的制剂中观察到的几何形状显著不同。速冻肺的肺泡壁是线性的;其他肺的肺泡壁是褶皱的。差异被证明是由于弹性蛋白的不完全固定。
Pulmonary alveolar geometry in histological sections prepared by quick-freezing the lung, with freeze-substitution of ice, fixation of the dry lung with osmium tetroxide in nonaqueous and nonpolar solvent and celloidin embedding is remarkably different from the geometry seen in preparations made by instilling aqueous aldehyde or osmium tetroxide solution in the airways. The alveolar walls in the quick-frozen lung are linear; those in the others are wrinkled. The difference was demonstrated to be due to incomplete fixation of elastin.