Membrane protein carboxyl methylation does not appear to be involved in the response of erythrocytes to cytoskeletal stress.
Membrane protein carboxyl methylation does not appear to be involved in the response of erythrocytes to cytoskeletal stress.
复制标题
膜蛋白羧基甲基化似乎不参与红细胞对细胞骨架应激的反应。
DOI：
10.1016/0006-291x(84)90390-5
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Clarke,S
中科院分区：
文献类型：
--
作者：
Barber,JR;Clarke,S
我们研究了人红细胞形态变化对膜细胞骨架蛋白羧甲基化修饰共价程度的影响。结果表明，细胞可能不利用羧甲基化来响应A23187、2，4-二硝基苯酚和氯丙嗪等药物引起的细胞骨架扰动，所有这些都会导致细胞形状的巨大变化。在细胞松弛素B的存在下，蛋白质的羧甲基化也保持不变，这可以防止细胞形状的这种变化。这些结果与完整红细胞中蛋白质甲基化反应的细胞骨架调节作用不一致。
We have investigated the effect of changes of human erythrocyte cell shape on the degree of covalent modification by carboxyl methylation of membrane cytoskeletal proteins. The results indicate that the cell probably does not utilize carboxyl methylation to respond to cytoskeletal perturbations caused by such agents as A23187, 2,4-dinitrophenol, and chlorpromazine, all of which are known to cause large changes in cell shape. Protein carboxyl methylation also remained unchanged in the presence of cytochalasin B, which prevents such changes in cell shape. These results are not consistant with a cytoskeletal regulatory role for protein methylation reactions in the intact erythrocyte.