Inhibition of intercellular gap junctional communication by alkyl ethers and its modulation by cAMP.
Inhibition of intercellular gap junctional communication by alkyl ethers and its modulation by cAMP.
复制标题
烷基醚对细胞间间隙连接通讯的抑制及其 cAMP 的调节。
作者：
Vítek Ja
：使用 V79 中国仓鼠细胞体外检查烷基醚（乙二醇、单甲醚、聚乙二醇 1,000 和聚乙二醇 6000）对细胞间间隙连接通讯 (IGJC) 的抑制作用。乙二醇和单甲醚对 IGJC 的抑制非常强烈，而其他药物对 IGJC 的抑制作用不显着。当用乙二醇和单甲醚这两种药物联合处理细胞时，IGJC 的抑制作用显着增加。这可能是增强效应而不是加成效应的结果。乙二醇的作用被二丁酰环磷酸腺苷（DbcAMP）拮抗。当同时用两种药物处理细胞时，这种效果最为强烈，而在其他实验组合中，效果较低，但也很显着。咖啡因与 DbcAMP 联合使用或单独使用均不会影响 IGJC。
: The inhibition of intercellular gap junctional communication (IGJC) by alkyl ethers (ethylene glycol, monomethyl ether, polyethylene glycol 1,000 and polyethylene glycol 6000) was examined using V79 Chinese hamster cells in vitro. Ethylene glycol and monomethyl ether inhibited IGJC very strongly, whilst the other agents inhibited IGJC only insignificantly. When the cells were treated with the combination of two agents, ethylene glycol and monomethyl ether, a significant increase in the inhibition of IGJC occurred. This was probably the result of potentiation rather than an addition effect. The effect of ethylene glycol was antagonized by dibutyryl cyclic adenosine monophosphate (DbcAMP). This effect was most intensive when the cells were treated with both agents at the same time and, in other experimental combinations, the effect was lower but also significant. Caffeine did not influence IGJC either in combination with DbcAMP or by itself.