Genetic epidemiology of hepatitis C virus throughout Egypt
Genetic epidemiology of hepatitis C virus throughout Egypt
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DOI：
10.1086/315786
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发表时间：
2000-09-01
影响因子：
6.4
通讯作者：
Thomas, DL
中科院分区：
文献类型：
--
作者：
Ray, SC;Arthur, RR;Thomas, DL
丙型肝炎病毒（HCV）感染是埃及的一个主要健康问题，其血清阳性率比美国高10-20倍。为了表征HCV基因型分布和多基因组区域基因型评估的一致性，从埃及15个地理位置不同的省份的献血者中获得标本。采用RT-PCR扩增5'端非编码区、核心/E1区和NS 5 B区，并进行限制性片段长度多态性（RFLP）分析和系统发育树构建。对190份标本中122份（64%）的5'端非编码区进行了扩增和RFLP分析：111份（91%）为4型，1份（1%）为1a型，1份（1%）为1b型，9份（7%）无法分型。核心/E1和NS 5 B区域的系统发育分析证实了基因型4的优势，并揭示了3个新亚型的证据，分离株之间的遗传距离分析与75-140年前引入的多个病毒株一致，并且在地理区域内未检测到聚类，表明自那时以来的某个时间广泛分散。
Hepatitis C virus (HCV) infection is a major health problem in Egypt, where the seroprevalence is 10-20-fold higher than that in the United States. To characterize the HCV genotype distribution and concordance of genotype assessments on the basis of multiple genomic regions, specimens were obtained from blood donors in 15 geographically diverse governorates throughout Egypt. The 5' noncoding, core/E1, and NS5B regions were amplified by reverse transcription-polymerase chain reaction and analyzed by both restriction fragment length polymorphism (RFLP) and phylogenetic tree construction. For the 5' noncoding region, 122 (64%) of 190 specimens were amplified and analyzed by RFLP: 111 (91%) were genotype 4, 1 (1%) was genotype 1a, 1 (1%) was genotype 1b, and 9 (7%) could not be typed. Phylogenetic analyses of the core/E1 and NS5B regions confirmed the genotype 4 preponderance and revealed evidence of 3 new subtypes, Analysis of genetic distance between isolates was consistent with the introduction of multiple virus strains 75-140 years ago, and no clustering was detected within geographic regions, suggesting widespread dispersion at some time since then.