Expression Analysis of Nudix Hydrolase Genes in Chrysanthemum lavandulifolium
Expression Analysis of Nudix Hydrolase Genes in Chrysanthemum lavandulifolium
复制标题
薰衣草Nudix水解酶基因的表达分析
DOI：
10.1007/s11105-011-0401-7
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发表时间：
2012-08-01
影响因子：
2.1
通讯作者：
Dai, Silan
中科院分区：
文献类型：
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作者：
Huang, He;Cao, Huawen;Dai, Silan
营养素水解酶与植物在非生物胁迫下的解毒过程有关，并调节抗病信号转导途径。研究菊花中8个营养素水解酶基因家族成员的表达模式和系统进化，将为菊花解毒途径的研究提供新的思路。本研究利用C.熏衣草表达序列标签（EST）数据库、EST剪接和cDNA末端的快速扩增。利用MEGA4.0软件对8个ClNUDX基因进行序列相似性分析，结果表明这些基因分属于植物营养素水解酶基因家族的7个亚家族。逆转录-聚合酶链反应分析表明，5个基因表现出器官特异性表达，这些基因包括ClNUDX 1，ClNUDX 2，ClNUDX 3，CINUDX 7和ClNUDX 8。其中，ClNUDX 1、ClNUDX 3、ClNUDX 7和ClNUDX 8由盐、干旱、热和冷胁迫诱导。ClNUDX 2只受盐和干旱的影响。ClNUDX 1和ClNUDX 2的转录水平也受到脱落酸和水杨酸的调节。克隆了ClNUDX 1的启动子CN 1 P。瞬时表达分析表明，CN 1 P能够驱动GUS报告基因的表达。因此，C.熏衣草表现出不同的表达模式响应不同的非生物胁迫。这些结果进一步阐明了对非生物胁迫的响应机制的认识。熏衣草
Nudix hydrolases are associated with detoxification processes in plants under abiotic stress and regulate disease resistance signal transduction pathways. Investigating expression patterns and phyletic evolution of eight Nudix hydrolase gene family members in Chrysanthemum lavandulifolium will provide insights into detoxification pathways. In this study, full-length open reading frames of eight Nudix hydrolase genes (ClNUDX1-8) were obtained using a C. lavandulifolium expressed sequence tag (EST) database, EST splicing, and rapid-amplification of cDNA ends. Sequence similarity analysis of the eight ClNUDXs using MEGA4.0 software revealed that these genes belonged to seven subfamilies of the plant Nudix hydrolase gene family. Reverse transcription-polymerase chain reaction analysis demonstrated that five genes exhibited organ-specific expression; these included ClNUDX1, ClNUDX2, ClNUDX3, CINUDX7, and ClNUDX8. Among those, ClNUDX1, ClNUDX3, ClNUDX7, and ClNUDX8 were induced by salt, drought, heat, and cold stresses. ClNUDX2 was only influenced by salt and drought. Transcription levels of both ClNUDX1 and ClNUDX2 were also regulated by abscisic acid and salicylic acid. The promoter of ClNUDX1, CN1P, was cloned. Transient expression analysis indicated that CN1P was capable of driving expression of the GUS reporter gene. Thus, it was concluded that different members of Nudix hydrolase genes in C. lavandulifolium demonstrated varied expression patterns in response to different abiotic stresses. These findings further elucidated knowledge on the response mechanisms to abiotic stresses in C. lavandulifolium.