Distinct innate immune responses in human macrophages and endothelial cells infected with shrew-borne hantaviruses.
Distinct innate immune responses in human macrophages and endothelial cells infected with shrew-borne hantaviruses.
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感染鼩传播汉坦病毒的人类巨噬细胞和内皮细胞出现独特的先天免疫反应。
DOI：
10.1016/j.virol.2012.08.004
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Song,Jin-Won
中科院分区：
文献类型：
--
作者：
Shin,OkSarah;Yanagihara,Richard;Song,Jin-Won
虽然汉坦病毒以前被认为是啮齿动物传播的病原体，但最近的研究表明，在进化上遥远的非啮齿动物宿主（包括鼩、鼹鼠和蝙蝠）中存在遗传上不同的汉坦病毒。人类对这些新发现的汉坦病毒的免疫反应尚不清楚。比较了鼠源汉坦病毒（HTNV）和非致病性前景希尔病毒（PHV）对鼠源汉坦病毒（HTNV）和临津江病毒（MJNV）和托塔帕拉亚姆病毒（TPMV）的天然免疫应答。与PHV和TPMV相比，HTNV或MJNV感染人巨噬细胞和内皮细胞后，从感染后第1天到第3天，触发了生产性病毒复制和抗病毒应答基因表达的上调。此外，HTNV，MJNV和TPMV感染导致从感染后第3天到第7天的促炎细胞因子的产生延长增加。相比之下，PHV感染未能诱导促炎反应。MJNV引起的先天免疫激活的独特模式表明它可能对人类具有致病性。
Although hantaviruses have been previously considered as rodent-borne pathogens, recent studies demonstrate genetically distinct hantaviruses in evolutionarily distant non-rodent reservoirs, including shrews, moles and bats. The immunological responses to these newfound hantaviruses in humans are unknown. We compared the innate immune responses to Imjin virus (MJNV) and Thottapalayam virus (TPMV), two shrew-borne hantaviruses, with that toward two rodent-borne hantaviruses, pathogenic Hantann virus (HTNV) and nonpathogenic Prospect Hill virus (PHV). Infection of human macrophages and endothelial cells with either HTNV or MJNV triggered productive viral replication and up-regulation of anti-viral responsive gene expression from day 1 to day 3 postinfection, compared with PHV and TPMV. Furthermore, HTNV, MJNV and TPMV infection led to prolonged increased production of pro-inflammatory cytokines from days 3 to 7 postinfection. By contrast, PHV infection failed to induce pro-inflammatory responses. Distinct patterns of innate immune activation caused by MJNV suggest that it might be pathogenic to humans.