Molecular characterization, spatial-temporal expression and magnetic response patterns of iron-sulfur cluster assembly1 (IscA1) in the rice planthopper, Nilaparvata lugens
Molecular characterization, spatial-temporal expression and magnetic response patterns of iron-sulfur cluster assembly1 (IscA1) in the rice planthopper, Nilaparvata lugens
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稻飞虱 Nilaparvata lugens 铁硫簇组装 1 (IscA1) 的分子特征、时空表达和磁响应模式
DOI：
10.1111/1744-7917.12546
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Pan Wei Dong
中科院分区：
文献类型：
--
作者：
Xu Jing Jing;Zhang Ying Chao;Wu Jian Qi;Wang Wei Hong;Li Yue;Wan Gui Jun;Chen Fa Jun;Sword Gregory A;Pan Wei Dong
磁感受机制已被提出为基于磁铁矿的化学自由基对和生物罗盘模型，其中可能涉及磁铁矿颗粒、隐花色素（Cry）或铁硫簇组件1（IscA1）。然而，人们对分子之间的关联知之甚少。本研究对飞虱Nilaparvata lugens中IscA1的分子特征及其在不同发育阶段、组织和磁场中的mRNA表达进行了研究。NlIscA1包含一个390 bp的开放阅读框，编码129个氨基酸，预测分子量为14.0 kDa，等电点为9.10。在预测的蛋白质中观察到了保守的 Fe-S 簇结合位点。系统发育分析表明NlIscA1聚类到昆虫的IscA1中。NlIscA1在迁徙过程中mRNA表达上调。 NlIscA1 mRNA在头、胸、腹部3个组织中均有表达，其中腹部表达量最高。对于大翅类迁徙型褐飞虱，NlIscA1和N的mRNA表达。 lugens cryptochrome1（Nlcry1）在5高斯和10高斯强度的磁场（相对于当地地磁场）下表达上调，而N. lugens cryptochrome2(Nlcry2)保持稳定。对于短肢类非洄游褐飞虱，不同磁场下NlIscA1、Nlcry1和Nlcry2 mRNA表达量未发现显着变化。这些发现初步揭示了NlIscA1和Nlcry1的表达对磁场表现出协调反应。它表明隐花色素和铁硫簇组装的假定磁敏分子之间存在一些潜在的关联。
The mechanisms of magnetoreception have been proposed as the magnetite‐based, the chemical radical‐pair and biocompass model, in which magnetite particles, the cryptochrome (Cry) or iron‐sulfur cluster assembly 1 (IscA1) may be involved. However, little is known about the association among the molecules. Here we investigated the molecular characterization and the mRNA expression of IscA1 in different developmental stages, tissues and magnetic fields in the migratory brown planthopper (BPH),Nilaparvata lugens.NlIscA1contains an open reading frame of 390 bp, encoding amino acids of 129, with the predicted molecular weight of 14.0 kDa and the isoelectric point of 9.10. Well‐conserved Fe‐S cluster binding sites were observed in the predicted protein. Phylogenetic analysis demonstratedNlIscA1to be clustered into the insect's IscA1.NlIscA1showed up‐regulated mRNA expression during the period of migration. The mRNA expression ofNlIscA1could be detected in all the three tissues of head, thorax and abdomen, with the highest expression level in the abdomen. For the macropterous migratoryNilaparvata lugens, mRNA expression ofNlIscA1andN. lugens cryptochrome1(Nlcry1) were up‐regulated under the magnetic fields of 5 Gauss and 10 Gauss in strength (vs. local geomagnetic field), whileN. lugens cryptochrome2(Nlcry2) remained stable. For the brachyterous non‐migratoryNilaparvata lugens, no significant changes were found in mRNA expression ofNlIscA1,Nlcry1andNlcry2among different magnetic fields. These findings preliminarily reveal that the expression ofNlIscA1andNlcry1exhibited coordinated responses to the magnetic field. It suggests some potential associations among the putative magneto‐sensitive molecules of cryptochrome and iron‐sulfur cluster assembly.