Lack of an association between environmental exposure to polychlorinated biphenyls and p,p′-DDE and DNA damage in human sperm measured using the neutral comet assay
Lack of an association between environmental exposure to polychlorinated biphenyls and p,p′-DDE and DNA damage in human sperm measured using the neutral comet assay
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DOI：
10.1093/humrep/deg508
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发表时间：
2003-12-01
影响因子：
6.1
通讯作者：
Altshul, L
中科院分区：
文献类型：
--
作者：
Hauser, R;Singh, NP;Altshul, L
背景技术背景：氯化有机化学品，如多氯联苯（PCB）、六氯苯（HCB）、二氯二苯三氯乙烷（DDT）和二氯二苯二氯乙烯（DDE，DDT最稳定的子化合物）是在大部分普通人群中发现的持久性亲脂性化合物。为了探索环境暴露于这些化合物与人类精子中DNA完整性改变有关的假设，对一对低生育力夫妇的212名男性伴侣进行了一项研究，这些夫妇被送到马萨诸塞州总医院男科实验室。方法：采用中性单细胞微凝胶电泳技术（彗星试验）检测精子DNA的完整性。VisComet图像分析软件用于测量彗星总长度、存在于彗星尾部的DNA比例以及尾部分布矩，这是长度和强度的综合度量。研究结果：在回归分析中，彗星试验参数与任何单个PCB同系物、PCB总和或p，p '-DDE之间没有统计学显著一致的关联。结论：这些结果表明，这些氯代有机化合物的成人水平和精子DNA损伤之间没有很强的关系，通过彗星试验测量。
BACKGROUND: Chlorinated organic chemicals, such as polychlorinated biphenyls (PCB), hexachlorobenzene (HCB), dichlorodiphenyl trichloroethane (DDT), and dichlorodiphenyl dichloroethene (DDE, the most stable daughter compound of DDT) are persistent lipophilic compounds found in a large portion of the general population. To explore the hypothesis that environmental exposure to these compounds is associated with altered DNA integrity in human sperm, a study of 212 male partners of a sub-fertile couple who presented to the Massachusetts General Hospital Andrology Laboratory was conducted. METHODS: The neutral single cell microgel electrophoresis assay (comet assay) was used to assess DNA integrity in sperm. VisComet image analysis software was used to measure total comet length, the proportion of DNA present in the comet tail, and tail distributed moment, an integrated measure of length and intensity. RESULTS: In the regression analyses, there were no statistically significant consistent associations between the comet assay parameters and any of the individual PCB congeners, sum of PCB, or p,p'-DDE. CONCLUSION: These results suggest that there are not strong relationships between adult levels of these chlorinated organic compounds and sperm DNA damage as measured by the comet assay.