OsATG10b, an Autophagosome Component, Is Needed for Cell Survival against Oxidative Stresses in Rice
OsATG10b, an Autophagosome Component, Is Needed for Cell Survival against Oxidative Stresses in Rice
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DOI：
10.1007/s10059-009-0006-2
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发表时间：
2009-01-01
影响因子：
3.8
通讯作者：
An, Gynheung
中科院分区：
文献类型：
--
作者：
Shin, Jun-Hye;Yoshimoto, Kohki;An, Gynheung
自噬降解有毒物质和旧的细胞器，并在真核细胞中吸收营养。虽然自噬的研究已经在其他真核细胞中报道，但其在植物中的功能尚未得到很好的阐明。我们分析了自噬相关的OsATG 10基因的作用。水稻ATG 10基因OsATG 10a和OsATG 10 b-具有75%的同源性，在所有器官中都有广泛表达。GUS检测结果表明，OsATG 10 b在幼嫩叶片的叶肉细胞和维管组织中表达量较高，但在老叶中表达量下降。我们在该基因中发现了T-DNA插入突变体。这些osatg 10 b突变体对高盐和甲基紫精（MV）处理敏感。单丹酰尸胺染色实验表明，自噬体的数量显着减少的突变体相比，野生型。此外，MV处理的突变体幼苗中氧化蛋白的量增加。这些结果表明OsATG 10 b在水稻细胞抗氧化胁迫的存活中起重要作用。
Autophagy degrades toxic materials and old organelles, and recycles nutrients in eukaryotic cells. Whereas the studies on autophagy have been reported in other eukaryotic cells, its functioning in plants has not been well elucidated. We analyzed the roles of OsATG10 genes, which are autophagy-related. Two rice ATG10 genes OsATG10a and OsATG10b - share significant sequence homology (about 75%), and were ubiquitously expressed in all organs examined here. GUS assay indicated that OsATG10b was highly expressed in the mesophyll cells and vascular tissue of younger leaves, but its level of expression decreased in older leaves. We identified T-DNA insertional mutants in that gene. Those osatg10b mutants were sensitive to treatments with high salt and methyl viologen (MV). Monodansylcadaverine-staining experiments showed that the number of autophagosomes was significantly decreased in the mutants compared with the WT. Furthermore, the amount of oxidized proteins increased in MV-treated mutant seedlings. These results demonstrate that OsATG10b plays an important role in the survival of rice cells against oxidative stresses.