Comprehensive and Sequential Gene Expression Analysis of Bone Healing Process Following Er:YAG Laser Ablation
Comprehensive and Sequential Gene Expression Analysis of Bone Healing Process Following Er:YAG Laser Ablation
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DOI：
10.1089/photob.2020.4833
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发表时间：
2020-12-21
影响因子：
1.8
通讯作者：
Aoki, Akira
中科院分区：
文献类型：
--
作者：
Shimohira, Tsuyoshi;Katagiri, Sayaka;Aoki, Akira
目的：本研究评估了全面和顺序的基因表达激光消融骨相比，在未经处理的control bone. Background：骨消融Er：YAG激光已显示出积极的影响，骨愈合，然而，发生在骨愈合的基因表达反应仍然不清楚。材料和方法：男性，10周龄Wistar大鼠的颅骨被烧蚀Er：YAG激光。在6，24和72小时，使用微阵列分析在激光消融骨和未处理的对照骨的基因表达进行了评价。结果：BCAR 1/p130 Cas（breast cancer anti-estrogen resistance 1/p130 Crk-associated substrate）基因表达水平逐渐升高，而P130 Cas基因表达水平逐渐升高。此外，根据京都基因百科全书和基因组途径分析，在6 h时Hippo信号传导途径的上游富集。F-actin mRNA表达也逐渐增加，而Hippo信号通路从6到24 h下调。骨形成相关的基因本体论（GO）的条款的富集从早期阶段观察到，而炎症相关的GO条款，基因集，和Nfkb1，Tnf和Il1b的mRNA表达逐渐丰富后24小时。结论：骨消融Er：YAG激光调节Bcar1和Actg1的表达，在骨组织中的机械传导的主要调节器。此外，在Er：YAG激光骨消融后72 h内，炎症逐渐增加。激光照射后立即影响与骨形成相关的基因表达。因此，Er：YAG激光照射引起的机械应力和生物学效应可能有助于激光消融骨组织中的伤口愈合。
Objective: This study evaluated the comprehensive and sequential gene expression in laser-ablated bone compared with that in nontreated control bone.Background: Bone ablation by Er:YAG laser has shown positive effects on bone healing; however, the gene expression responses that occur during bone healing remain unclear.Materials and methods: The calvarial bone of male, 10-week-old Wistar rats was ablated by Er:YAG laser. Gene expression in the laser-ablated bone and nontreated control bone was evaluated at 6, 24, and 72 h using microarray analysis. Messenger RNA (mRNA) expression levels were validated by quantitative reverse transcription-polymerase chain reaction.Results: Gene expression of BCAR1/p130Cas (breast cancer anti-estrogen resistance 1/p130 Crk-associated substrate), a mechanotransducer, was gradually increased. Additionally, upstream of the Hippo signaling pathway was enriched according to Kyoto Encyclopedia of Genes and Genomes pathway analysis at 6 h. F-actin mRNA expression was also gradually increased, whereas the Hippo signaling pathway was downregulated from 6 to 24 h. Enrichment of bone formation-related Gene Ontology (GO) terms was observed from an early stage, whereas inflammation-related GO terms, gene sets, and mRNA expression of Nfkb1, Tnf, and Il1b were gradually enriched after 24 h.Conclusions: Bone ablation by Er:YAG laser regulated the expression of Bcar1 and Actg1, the main regulators of mechanotransduction in the bone tissue. Additionally, inflammation was gradually increased up to 72 h following bone ablation with Er:YAG laser. Laser influences the expression of genes associated with bone formation immediately after irradiation. Therefore, mechanical stress and the biological effects caused by Er:YAG laser irradiation potentially contribute to wound healing in the laser-ablated bone tissue.