Structural transition in aqueous lipid/bile salt [DPPC/NaDC] supramolecular aggregates: SANS and DLS study
Structural transition in aqueous lipid/bile salt [DPPC/NaDC] supramolecular aggregates: SANS and DLS study
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DOI：
10.1016/j.chemphys.2013.05.014
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发表时间：
2013-10-16
期刊：
影响因子：
2.3
通讯作者：
Lombardo, D.
中科院分区：
文献类型：
--
作者：
Kiselev, M. A.;Janich, M.;Lombardo, D.
小角中子散射 (SANS) 和动态光散射 (DLS) 用于研究 T = 60 摄氏度时脱氧胆酸钠 (NaDC)-磷脂酰胆碱系统中的不同聚集状态。所研究的聚集体的尺寸和形状作为 NaDC 胆盐浓度的函数（在 6 mM 的恒定 DPPC 浓度下）表明所生成的聚集体的尺寸和形态对 NaDC 胆盐的相对量有很强的依赖性。更具体地说，胆盐的大占据面积诱导空间相互作用，促进向各种超分子结构的转变，从椭圆体囊泡、带状结构，到在大量 10 mM NaDC 胆盐下的最终球形混合胶束。所获得的结果为理解水性脂质胆盐混合物中不同拓扑的形成提供了重要的见解，并激发了适合技术应用的脂质体重构-溶解过程的新途径。 (C) 2013 Elsevier B.V. 保留所有权利。
Small angle neutron scattering (SANS) and dynamic light scattering (DLS) were used to study different aggregation states in sodium deoxycholate (NaDC)-phosphatidylcholine systems at T = 60 degrees C. Size and shape of the aggregates investigated as a function of the NaDC bile salt concentration (at the constant DPPC concentration of 6 mM) indicate a strong dependence of the size and morphology of the generated aggregates on the relative amount of NaDC bile salt. More specifically large occupied area of the bile salt induces a steric interaction which promotes the transition toward a variety of supramolecular structures ranging from ellipsoidal vesicles, ribbon-like structures, up to final spherical mixed micelles at the large amount of bile salt of 10 mM NaDC. The findings of the obtained results give important insight for understanding the formation of different topologies in aqueous lipid-bile salt mixtures as well as stimulate new routes for liposome reconstitution-solubilisation processes suitable for technological applications. (C) 2013 Elsevier B. V. All rights reserved.