Exposure to benzo[a]pyrene impairs decidualization and decidual angiogenesis in mice during early pregnancy
Exposure to benzo[a]pyrene impairs decidualization and decidual angiogenesis in mice during early pregnancy
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暴露于苯并[a]芘会损害妊娠早期小鼠的蜕膜化和蜕膜血管生成
DOI：
10.1016/j.envpol.2016.11.029
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发表时间：
2017-03-01
影响因子：
8.9
通讯作者：
Chen, Xuemei
中科院分区：
文献类型：
--
作者：
Li, Xueyan;Shen, Cha;Chen, Xuemei
苯并[a]芘(BaP)是一种普遍存在的环境持久性有机污染物，是一种众所周知的内分泌干扰物。暴露于BaP会改变女性体内的类固醇平衡。子宫内膜蜕膜化和蜕膜血管生成是早期妊娠胚胎着床和妊娠维持的关键事件，并受类固醇的调节。然而，BaP对蜕膜化的影响尚不清楚。本研究旨在探讨BaP对妊娠小鼠蜕膜化和蜕膜血管生成的影响。结果显示，与对照组相比，BaP治疗组的子宫较小，大小不均。对照组子宫内有人工蜕膜化现象，而BaP处理组蜕膜化反应减弱。BaP可显著降低雌二醇、孕酮及其受体ER和PR的水平。BaP治疗后蜕膜化相关因子FOX01、HoxA10和BMP2的表达发生改变。BaP可降低蜕膜血管生成基因CD34的表达，提示BaP可抑制蜕膜血管生成。此外，BaP诱导血管内皮生长因子A的下调。这些数据表明，口服BaP会损害蜕膜和蜕膜血管的生成。本研究结果为早孕期苯并苯的母体生殖毒性提供了实验数据，对全面评价苯并苯对人类生殖健康的危害具有重要意义。(C)2016爱思唯尔有限公司。保留所有权利。
Benzo[a]pyrene (BaP) is a ubiquitous environmental persistent organic pollutant and a well-known endocrine disruptor. BaP exposure could alter the steroid balance in females. Endometrium decidualization and decidual angiogenesis are critical events for embryo implantation and pregnancy maintenance during early pregnancy and are modulated by steroids. However, the effect of BaP on decidualization is not clear. This study aimed to explore the effects of BaP on decidualization and decidual angiogenesis in pregnant mice. The result showed that the uteri in the BaP-treated groups were smaller and exhibited an uneven size compared with those in the control group. Artificial decidualization was detected in the uteri of the controls, but weakened decidualization response was observed in the BaP-treated groups. BaP significantly reduced the levels of estradiol, progesterone, and their cognate receptors ER and PR, respectively. The expression of several decidualization-related factors, including FOX01, HoxA10, and BMP2, were altered after BaP treatment. BaP reduced the expression of cluster designation 34 (CD34), which indicated that the decidual angiogenesis was inhibited by BaP treatment. In addition, BaP induced the downregulation of vascular endothelial growth factor A. These data suggest that oral BaP ingestion compromised decidualization and decidual angiogenesis. Our results provide experimental data for the maternal reproductive toxicity of BaP during early pregnancy, which is very important for a comprehensive risk assessment of BaP on human reproductive health. (C) 2016 Elsevier Ltd. All rights reserved.