Surface Engineering of Nano-Fibrous Poly(L-Lactic Acid) Scaffolds via Self-Assembly Technique for Bone Tissue Engineering
Surface Engineering of Nano-Fibrous Poly(L-Lactic Acid) Scaffolds via Self-Assembly Technique for Bone Tissue Engineering
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DOI：
10.1166/jbn.2005.013
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发表时间：
2005-03-01
影响因子：
2.9
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Liu, Xiaohua;Smith, Laura;Ma, Peter X.
支架的结构设计和表面性能是组织工程中的重要问题。多孔支架提供了容纳细胞并引导其生长的环境。支架的表面性质影响细胞的附着、增殖，最终影响新生组织的再生。本研究采用本课题组开发的一种新型热诱导相分离方法，制备了具有纳米纤维结构的高孔L-乳酸(PLLA)支架，以模拟天然胶原的结构。采用静电逐层自组装技术将明胶负载到纳米纤维聚乳酸(nF-PLLA)支架表面。采用ATR-FTIR、XPS、扫描电子显微镜和接触角等测试手段对其表面组成、形貌和性能进行了分析。凝胶量受聚电解质双层膜的组装数量控制，在前两层膜之后，明胶含量随膜层数的增加而线性增加。表面改性后，接触角减小，亲水性得到改善。将MC3T3-E1成骨祖细胞培养在表面修饰的薄膜和支架上。细胞接种后4h和2 4h，表面修饰的NF-PLLA膜上的细胞数显著高于对照组，且随着双层数的增加而增加。此外，成骨细胞在表面修饰的NF-PLLA支架上的增殖速度更快，分布更均匀。这些结果表明，静电自组装是一种有效的三维支架表面修饰技术，表面修饰后的纳米纤维支架有利于细胞的黏附和增殖。
The architectural design and surface properties of scaffolds are important in tissue engineering. The porous scaffolds provide the environment to accommodate cells and guide their growth. The surface nature of the scaffolds affect cell attachment, proliferation, and ultimately neo tissue regeneration. In this work, a highly porous Poly(L-lactic acid) (PLLA) scaffold with nano-fibrous architecture has been fabricated to mimic the structure of natural collagen using a novel thermally induced phase separation method developed in our group. The electrostatic layer-by-layer self-assembly technique was applied to incorporate gelatin onto the surface of nano-fibrous PLLA (NF-PLLA) scaffolds. The surface composition, morphology, and properties were examined using ATR-FTIR, XPS, SEM and contact angle measurements. The amount of gelatin on a NF-PLLA film was controlled by the number of assembled polyelectrolyte bilayers, and increased linearly with the bilayer number after the first two bilayers. The contact angle decreased upon surface modification, indicating improvement in hydrophilicity. MC3T3-E1 osteoprogenitor cells were cultured on these surface-modified films and scaffolds. The cell number on the surface-modified NF-PLLA film was significantly higher than that on the control 4 h and 24 h after cell seeding, and increased with the number of bilayers. Furthermore, the osteoblasts proliferated at a higher rate and were more evenly distributed on surface-modified NF-PLLA scaffolds than on the control scaffolds. These results demonstrated that the electrostatic self-assembly was a powerful technique to modify three dimensional scaffold surfaces, and the surface-modified nano-fibrous scaffolds were advantageous for cell adhesion and proliferation.