Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila
Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila
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DOI：
10.1007/s002990100320
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发表时间：
2001-03-01
影响因子：
6.2
通讯作者：
Aimi, N
中科院分区：
文献类型：
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作者：
Saito, K;Sudo, H;Aimi, N
喜树碱衍生物在临床上用作抗肿瘤生物碱。喜树碱及其相关化合物目前通过从完整植物中提取获得，但转化的植物细胞培养物可能是生产的替代来源。我们建立了发根农杆菌15834菌株转化乌藨子的毛状根培养体系。这种毛状根培养物生长良好，在液体培养5周期间增加了16倍，并且它产生喜树碱作为主要生物碱高达0.1%/细胞干重。有趣的是，不仅毛状根细胞含有喜树碱，而且培养基也积累了大量的喜树碱。培养基中的喜树碱含量通过存在吸收喜树碱的聚苯乙烯树脂（Diaion HP-20）而增加。喜树碱易于从树脂中回收。我们的方法是通过体外细胞培养生产喜树碱的最可行和商业上适用的方法。
Camptothecin derivatives are used clinically as anti-tumor alkaloids. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but transformed plant cell cultures may be an alternative source of production. We have established a hairy root culture of Ophiorriza pumila (Rubiaceae) transformed by Agrobacterium rhizogenes strain 15834. This hairy root culture grew well, increasing by 16-fold during 5 weeks in liquid culture, and it produced camptothecin as a main alkaloid up to 0.1% per dry weight of the cells. Interestingly, not only the hairy root cells contained camptothecin, but the culture medium also accumulated substantial amounts. Camptothecin content in the medium was increased by the presence of a polystyrene resin (Diaion HP-20) that absorbed camptothecin. Camptothecin was easily recovered from the resin. Our method is the most feasible and commercially applicable way to produce camptothecin by in vitro cell culture.