Lactoferrin restrains allergen-induced pleurisy in mice.
Lactoferrin restrains allergen-induced pleurisy in mice.
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乳铁蛋白可抑制小鼠过敏原诱发的胸膜炎。
DOI：
10.1007/s00011-012-0522-y
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kruzel,MarianL
中科院分区：
文献类型：
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作者：
Zimecki,Michał;Artym,Jolanta;Kocięba,Maja;Kaleta-Kuratewicz,Katarzyna;Kruzel,MarianL
本研究的目的是评估乳铁蛋白（LF），一种天然的免疫调节剂，抑制过敏原诱导的小鼠胸膜炎的效用。用50 μg卵清蛋白（OVA）腹腔内免疫小鼠，14天后胸膜腔内注射12.5 μg卵清蛋白诱发胸膜炎。LF给予前24和3小时的过敏反应elicitation.MethodsThe胸膜渗出液中的细胞因子水平的免疫测定。使用Giemsa和May-Grünwald试剂对血液和胸膜渗出液涂片进行染色，并进行组织学审查。肺切片用伊红和苏木精染色组织学evaluation.ResultsLactoferrin显着降低表现致敏小鼠模型中的OVA诱导的胸膜炎。特别是，血液和胸膜渗出液中嗜酸性粒细胞的百分比显著降低。肺组织学分析显示，LF减少了病理学病变的发展，如肺水肿，弥漫性肺泡出血和含铁血黄素沉着症，这些病变在注射诱发剂量的OVA后在肺中发现。LF也降低了IL-5分泌到pleural fluid.ConclusionsThis的水平是第一次证明，LF显着降低抗原特异性胸膜炎在致敏小鼠模型。
ObjectivesThe aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.Material and subjectsBALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.TreatmentMice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.MethodsThe cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.ResultsLactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.ConclusionsThis is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.