The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors.
The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors.
复制标题
荧光相关光谱的使用来表征荧光标记的G蛋白偶联受体的分子迁移率。
DOI：
10.1042/bst20150285
复制
发表时间：
2016-04-15
影响因子：
3.9
通讯作者：
Hill SJ
中科院分区：
文献类型：
--
作者：
Kilpatrick LE;Hill SJ
活细胞的膜已被证明是高度组织成不同的微区，这具有空间和时间的影响膜结合受体和它们的信号伴侣的相互作用作为复合物。荧光相关光谱（FCS）是一种具有单细胞灵敏度的技术，其揭示了活细胞的小质膜区域内的荧光标记受体、配体或信号传导复合物的分子动力学。本文综述了FCS用于G蛋白偶联受体（GPCRs）扩散和浓度的真实的实时定量检测的应用，主要是为了深入了解配体-受体相互作用和信号复合物的分子组成。此外，我们还记录了光子计数直方图（PCH）分析的使用，以研究分子亮度（ε）的变化如何成为荧光标记部分分子质量变化的敏感指标。
The membranes of living cells have been shown to be highly organized into distinct microdomains, which has spatial and temporal consequences for the interaction of membrane bound receptors and their signalling partners as complexes. Fluorescence correlation spectroscopy (FCS) is a technique with single cell sensitivity that sheds light on the molecular dynamics of fluorescently labelled receptors, ligands or signalling complexes within small plasma membrane regions of living cells. This review provides an overview of the use of FCS to probe the real time quantification of the diffusion and concentration of G protein-coupled receptors (GPCRs), primarily to gain insights into ligand–receptor interactions and the molecular composition of signalling complexes. In addition we document the use of photon counting histogram (PCH) analysis to investigate how changes in molecular brightness (ε) can be a sensitive indicator of changes in molecular mass of fluorescently labelled moieties.