The Interaction between LYVE-1 with Hyaluronan on the Cell Surface May Play a Role in the Diversity of Adhesion to Cancer Cells
The Interaction between LYVE-1 with Hyaluronan on the Cell Surface May Play a Role in the Diversity of Adhesion to Cancer Cells
复制标题
LYVE-1 与细胞表面透明质酸之间的相互作用可能在癌细胞粘附的多样性中发挥作用
DOI：
10.1371/journal.pone.0063463
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发表时间：
2013-05-22
期刊：
影响因子：
3.7
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Du, Yan;Liu, Hua;Gao, Feng
透明质酸（HA）是一种简单的双糖单位，可以降解，被认为是细胞外基质的主要成分，具有广泛的生物学功能。近年来，在肿瘤细胞表面发现了HA。根据以往的报道，肿瘤细胞表面HA含量的不同与淋巴结转移密切相关，但介导这一过程的机制尚不清楚。本研究旨在研究HA在肿瘤细胞表面的含量，并分析HA与其淋巴管内皮受体（LYVE-1）相互作用引起的细胞粘附变化。我们通过颗粒排斥、免疫荧光和流式细胞术实验筛选并观察到HS-578 T乳腺细胞上HA含量高，MCF-7乳腺细胞上HA含量低。LYVE-1（淋巴管特异性HA受体）的表达在细胞静态粘附和动态平行板流动室实验中与我们先前的报道一致，并通过HA增强HA高HS-578 T细胞与COS-7 LYVE-1（+）的粘附。MCF-7乳腺细胞表面几乎不含HA;然而，我们的结果显示MCF-7细胞与COS-7 LYVE-1（+）和COS-7 LYVE-1（-）细胞之间的粘附差异很小。关于HS-578 T细胞或MCF-7细胞对SVEC 4 -10细胞的粘附，观察到类似的结果。此外，我们首次观察到高转移肿瘤细胞的细胞表面HA含量丰富，并且我们可视化了HA电缆结构的交联，这可能激活淋巴管内皮细胞上的LYVE-1，促进肿瘤粘附。综上所述，高低细胞表面HA含量的肿瘤细胞通过与LYVE-1的相互作用导致粘附差异。
Hyaluronan (HA), a simple disaccharide unit, can polymerize and is considered a primary component of the extracellular matrix, which has a wide range of biological functions. In recent years, HA was found on the surface of tumor cells. According to previous reports, differing HA content on the cell surface of tumor cells is closely related to lymph node metastases, but the mechanisms mediating this process remained unclear. This research intended to study the surface content of HA on tumor cells and analyze cell adhesive changes caused by the interaction between HA and its lymphatic endothelial receptor (LYVE-1). We screened and observed high HA content on HS-578T breast cells and low HA content on MCF-7 breast cells through particle exclusion, immunofluorescence and flow cytometry experiments. The expression of LYVE-1, the lymph-vessel specific HA receptor, was consistent with our previous report and enhanced the adhesion of HAhigh-HS-578T cells to COS-7LYVE-1(+) through HA in cell static adhesion and dynamic parallel plate flow chamber experiments. MCF-7 breast cells contain little HA on the surface; however, our results showed little adhesion difference between MCF-7 cells and COS-7LYVE-1(+) and COS-7LYVE-1(−) cells. Similar results were observed concerning the adhesion of HS-578T cells or MCF-7 cells to SVEC4-10 cells. Furthermore, we observed for the first time that the cell surface HA content of high transfer tumor cells was rich, and we visualized the cross-linking of HA cable structures, which may activate LYVE-1 on lymphatic endothelial cells, promoting tumor adhesion. In summary, high-low cell surface HA content of tumor cells through the interaction with LYVE-1 leads to adhesion differences.