Human milk oligosaccharides inhibit the adhesion to Caco-2 cells of diarrheal pathogens:: Escherichia coli, Vibrio cholerae, and Salmonella fyris
Human milk oligosaccharides inhibit the adhesion to Caco-2 cells of diarrheal pathogens:: Escherichia coli, Vibrio cholerae, and Salmonella fyris
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DOI：
10.1203/01.pdr.0000200805.45593.17
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发表时间：
2006-03-01
影响因子：
3.6
通讯作者：
Orazio, G
中科院分区：
文献类型：
--
作者：
Coppa, GV;Zampini, L;Orazio, G
母乳喂养的儿童与奶瓶喂养的儿童相比，急性胃肠炎的发病率较低，这是由于母乳中存在几种抗感染因子。本研究的目的是研究人乳低聚糖预防与一些常见致病菌相关的感染的能力。采用凝胶过滤法分离人乳中的寡糖，并通过薄层色谱和高效阴离子交换色谱进行表征。获得的级分分别含有1）酸性低聚糖、2）中性高分子量低聚糖和3）中性低分子量低聚糖。进行实验以研究寡糖抑制三种肠道微生物（肠致病性大肠杆菌血清型0119、霍乱弧菌和沙门氏菌）对分化的Caco-2细胞的粘附的能力。研究表明，酸性部分对所有三种致病菌株都有抗粘附作用（抑制程度不同）。中性高分子量组分对E. coli 0119和霍乱弧菌，而对S. fyris;中性低分子量组分对E. coli 0119和S. fyris而不是霍乱弧菌。我们的研究结果表明，人乳低聚糖不仅能抑制常见病原体如大肠杆菌对上皮细胞的粘附，大肠杆菌，也首次发现了其他侵袭性细菌如霍乱弧菌和沙门氏菌。fyris。因此，低聚糖是母乳中含有的重要防御因子之一，可预防母乳喂养婴儿的急性腹泻感染。
Breast-fed children, compared with the bottle-fed ones, have a lower incidence of acute gastroenteritis due to the presence of several antiinfective factors in human milk. The aim of this work is to study the ability of human milk oligosaccharides to prevent infections related to some common pathogenic bacteria. Oligosaccharides of human milk were fractionated by gel-filtration and characterized by thin-layer chromatography and high-performance anion exchange chromatography. Fractions obtained contained, respectively, 1) acidic oligosaccharides, 2) neutral high-molecular-weight oligosaccharides, and 3) neutral low-molecular-weight oligosaccharides. Experiments were carried out to study the ability of oligosaccharides in inhibiting the adhesion of three intestinal microorganisms (enteropathogenic Escherichia coli serotype 0 119, Vibrio cholerae, and Salmonella fyris) to differentiated Caco-2 cells. The study showed that the acidic fraction had an antiadhesive effect on the all three pathogenic strains studied (with different degrees of inhibition). The neutral high-molecular-weight fraction significantly inhibited the adhesion of E. coli 0 119 and V. cholerae, but not that of S. fyris; the neutral low-molecular-weight fraction was effective toward E. coli 0119 and S. fyris but not V. cholerae. Our results demonstrate that human milk oligosaccharides inhibit the adhesion to epithelial cells not only of common pathogens like E. coli but also for the first time of other aggressive bacteria as V. cholerae and S. fyris. Consequently, oligosaccharides are one of the important defensive factors contained in human milk against acute diarrheal infections of breast-fed infants.