ARBITRARILY PRIMED PCR DNA-FINGERPRINTING OF ESCHERICHIA-COLI O157/H7 STRAINS BY USING TEMPLATES FROM BOILED CULTURES
ARBITRARILY PRIMED PCR DNA-FINGERPRINTING OF ESCHERICHIA-COLI O157/H7 STRAINS BY USING TEMPLATES FROM BOILED CULTURES
复制标题
DOI：
10.1128/jcm.33.6.1534-1536.1995
复制
发表时间：
1995-06-01
影响因子：
9.4
通讯作者：
BERG, DE
中科院分区：
文献类型：
--
作者：
MADICO, G;AKOPYANTS, NS;BERG, DE
引物PCR允许以高灵敏度和效率区分遗传上不同的细菌菌株。我们报告说，信息丰富，可重复的任意引物PCR图谱可以从大肠杆菌O157：H7菌株通过使用煮沸的固定相培养，而不需要耗时的苯酚提取。这种简单的模板制备程序在大型流行病学研究中特别有用，因为需要对许多菌株进行分型。
Arbitrarily primed PCR allows genetically different bacterial strains to be distinguished with great sensitivity and efficiency. We report that informative, reproducible arbitrarily primed PCR profiles can be obtained from Escherichia coli O157:H7 strains by using boiled stationary-phase cultures, without the need for time-consuming phenol extraction. This simple template preparation procedure should be especially useful in large epidemiologic studies when many strains must be typed.