Differentiation of Midbrain from Human iPS Cells
Differentiation of Midbrain from Human iPS Cells
复制标题
中脑与人类 iPS 细胞的分化
DOI：
10.1007/978-1-0716-1495-2_8
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Akamatsu Wado
中科院分区：
文献类型：
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作者：
Ishikawa Kei-Ichi;Nonaka Risa;Akamatsu Wado
人类诱导多能干细胞（iPS）为分析疾病机制和药物筛选提供了强有力的手段，特别是对于神经系统疾病，考虑到难以获得活的病理组织。中脑黑质的多巴胺能神经元在帕金森病中主要受到影响，但不可能从活体患者中获得和分析存活的多巴胺能神经元。这个问题可以通过从人iPS细胞诱导多巴胺能神经元来克服。在这里，我们描述了一种有效的方法，用于将人iPS细胞分化为中脑多巴胺能神经元。该方案对于更深入地了解疾病和开发新的治疗方法具有价值。
Human-induced pluripotent stem (iPS) cells provide a powerful means for analyzing disease mechanisms and drug screening, especially for neurological diseases, considering the difficulty to obtain live pathological tissue. The midbrain dopaminergic neurons of the substantia nigra are mainly affected in Parkinson’s disease, but it is impossible to obtain and analyze viable dopaminergic neurons from live patients. This problem can be overcome by the induction of dopaminergic neurons from human iPS cells. Here, we describe an efficient method for differentiating human iPS cells into midbrain dopaminergic neurons. This protocol holds merit for obtaining a deeper understanding of the disease and for developing novel treatments.