Characterization of a 32 μm diameter carbon fiber electrode for in vivo fast-scan cyclic voltammetry
Characterization of a 32 μm diameter carbon fiber electrode for in vivo fast-scan cyclic voltammetry
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DOI：
10.1016/j.jneumeth.2012.09.011
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发表时间：
2012-11-15
影响因子：
3
通讯作者：
Yavich, Leonid
中科院分区：
文献类型：
--
作者：
Chadchankar, Heramb;Yavich, Leonid
碳纤维电极（CFE）由于其优异的电化学性能和生物相容性，常用于体内检测儿茶酚胺。快速扫描循环伏安法（FSCV）结合CFES允许检测儿茶酚胺，如多巴胺（DA）具有高特异性和可靠性。然而，神经科学的进步不断要求传感器具有更高的灵敏度和选择性。本研究调查了未经处理的CFE的直径为32 μ m，暴露长度为300 μ m的检测DA使用FSCV。尽管工作电极的面积较大，我们观察到的背景电流相比，常用的CFE的7 μ m直径和100 μ m的暴露长度只有一个小的增加。32 μ mCFE的灵敏度是7 μ mCFE的9倍。这些较大的电极表现出良好的线性和6倍高的信噪比比7 μ m CFEs在体外。32 μ m CFE对DA的选择性明显优于7 μ m CFE对3，4-二羟基苯乙酸的选择性，对抗坏血酸和高香草酸的选择性与7 μ m CFE相似。在体外和体内测试中，电极显示出良好的时间分辨率和电化学稳定性。(C)2012爱思唯尔有限公司版权所有。
Carbon fiber electrodes (CFE) are commonly used for in vivo detection of catecholamines due to their excellent electrochemical properties and biocompatibility. Fast-scan cyclic voltammetry (FSCV) combined with CFEs permits the detection of catecholamines such as dopamine (DA) with high specificity and reliability. However, advances in neuroscience constantly demand sensors with greater sensitivities and selectivities. This study investigated an untreated CFE of 32 mu m diameter and 300 mu m exposed length for detection of DA using FSCV. Despite the larger area of the working electrode, we observed only a small increase in the background current in comparison with the commonly used CFE of 7 mu m diameter and 100 mu m exposed length. The sensitivity of the 32 mu m CFE was 9 times greater than that of the 7 mu m CFE. These larger electrodes exhibited good linearity and a 6 fold higher signal-to-noise ratio than 7 mu m CFEs in vitro. The 32 mu m CFE showed significantly better selectivity for DA in preference to 3,4-dihydroxyphenylacetic acid than the 7 mu m CFE and similar selectivity to the 7 mu m CFE for ascorbic and homovanillic acid. The electrodes displayed good temporal resolution and electrochemical stability in both in vitro and in vivo tests. (C) 2012 Elsevier B.V. All rights reserved.