Genomic alterations in primary metaphase comparative genomic cutaneous melanomas detected by hybridization with laser capture or manual microdisSsection: 6p gains may predict poor outcome
Genomic alterations in primary metaphase comparative genomic cutaneous melanomas detected by hybridization with laser capture or manual microdisSsection: 6p gains may predict poor outcome
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DOI：
10.1016/j.cancergencyto.2004.06.004
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发表时间：
2005-02-01
影响因子：
--
通讯作者：
Kaneko, Y
中科院分区：
文献类型：
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作者：
Namiki, T;Yanagawa, S;Kaneko, Y
为了阐明基因组改变与临床和组织学特征的相关性，我们对20个原发性皮肤黑色素瘤（通过激光捕获或手动显微切割获得）和16个黑色素瘤细胞系进行了中期比较基因组杂交分析。肢端黑色素瘤（AM）和非AM之间的平均畸变数没有差异，尽管AM中5q和11q13的增加比非AM更频繁（P = 0.05），10q丢失比非AM更少（P = 0.01）。虽然肿瘤厚度被认为是临床表达的可测量估计值，但按肿瘤厚度分类的4组中畸变的平均数量无差异。虽然大多数畸变在4组中均匀分布，但仅在最厚的肿瘤中发现6p增益。6p或1q增益的患者的总生存率低于无增益的患者（P = 0.0002或P = 0.013）。虽然Iq、2q、3p、3q、7q、20p和20q的增加在细胞系中比在原发性肿瘤中更频繁（P < 0.01），但6q、9p、10p和10q的丢失在细胞系和原发性肿瘤中同样发现。目前的研究表明，染色体畸变已经发生在较薄的肿瘤，6p和1q的增益可能是一个预后因素。(C)2005年爱思唯尔公司All rights reserved.
To clarify the correlation of genomic alterations with clinical and histological features, we performed metaphase comparative genomic hybridization analysis on 20 primary cutaneous melanomas, which were obtained by laser capture or manual microdissection, and 16 melanoma cell lines. There were no differences in the average number of aberrations between acral melanomas (AM) and non-AM, although gains of 5q and 11q13 were more frequent (P = 0.05) and 10q loss was less frequent (P = 0.01) in AM than in non-AM. Although tumor thickness is considered a measurable estimate of clinical expression, there were no differences in the average number of aberrations among 4 groups, classified by thickness of the tumor. While the majority of aberrations were equally distributed among the 4 groups, 6p gains were found only in the thickest tumors. Patients with 6p or 1q gains had a lower overall survival rate than those without them (P = 0.0002 or P = 0.013). While gains of Iq, 2q, 3p, 3q, 7q, 20p, and 20q were more frequent in the cell lines than in the primary tumors (P < 0.01), losses of 6q, 9p, 10p, and 10q were equally found in both cell lines and primary tumors. The present study showed that chromosomal aberrations had already occurred in the thinner tumors, and that 6p and 1q gains maybe a prognostic factor. (C) 2005 Elsevier Inc. All rights reserved.