Glycyrrhiza polysaccharide doped the conducting polymer PEDOT hybrid-modified biosensors for the ultrasensitive detection of microRNA.
Glycyrrhiza polysaccharide doped the conducting polymer PEDOT hybrid-modified biosensors for the ultrasensitive detection of microRNA.
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DOI：
10.1016/j.aca.2020.09.061
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发表时间：
2020-10
影响因子：
6.2
通讯作者：
Hao Wang;Haitao Lü;Lili Yang;Zhiling Song;N. Hui
中科院分区：
文献类型：
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作者：
Hao Wang;Haitao Lü;Lili Yang;Zhiling Song;N. Hui
生物传感器在精确定位和快速检测人类疾病生物标志物方面具有巨大的革命性潜力。然而，非特异性蛋白质吸附相互作用和表面污染的最小化是其在复杂介质中的应用的关键。本文采用电化学方法将聚（3，4-亚乙基二氧噻吩）（PEDOT）与甘草多糖（GPS）共聚，构建了甘草多糖/PEDOT界面。亲水性PEDOT/GPS可以减少生物蛋白大分子的干扰和非特异性吸附，这一点已经通过电化学和荧光表征得到了验证。随后将金纳米颗粒（AuNPs）修饰到PEDOT/GPS表面以附着含有巯基的生物大分子。MicroRNA是一种很有前途的遗传疾病的生物标志物，它被用作检测模型。由于PEDOT/GPS的稳定性和GPS/AuNPs的高生物相容性，基于PEDOT/GPS/AuNPs的生物传感器表现出优异的传感性能，例如宽的检测范围（0.01 nM-10 nM），低的检测限（300 fM）和高的再现性，显示出巨大的医学应用潜力。
Biosensors have tremendous revolutionary potential for the precise location and rapid detection of biomarkers for human disease. However, the minimization of nonspecific protein adsorption interactions and surface contamination is critical for their application in complex media. We report herein, the antifouling interface was constructed by electrochemical copolymerization of poly (3,4-ethylenedloxythlophene) (PEDOT) and glycyrrhiza polysaccharide (GPS). Hydrophilic PEDOT/GPS can reduce the interference and nonspecific adsorption of biological protein macromolecules, which has been verified by electrochemical and fluorescent characterization. Gold nanoparticles (AuNPs) were subsequently modified onto PEDOT/GPS surface to attach biomacromolecules containing thiol groups. MicroRNA, the promising biomarker of a large numbers of genetic diseases, was used as the testing model. Due to the robust antifouling capability of PEDOT/GPS as well as high biocompatibility of GPS/AuNPs, the fabricated biosensor based on PEDOT/GPS/AuNPs demonstrated excellent sensing performance, such as a wide detection range (0.01 nM–10 nM), a low detection limit (300 fM) and high reproducibility, showing great potential of medical applications.