The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C-elegans
The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C-elegans
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DOI：
10.1016/j.ydbio.2006.04.001
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发表时间：
2006-07-15
影响因子：
2.7
通讯作者：
Okkema, Peter G.
中科院分区：
文献类型：
--
作者：
Chowdhuri, Sinchita Roy;Crum, Tanya;Okkema, Peter G.
梭秀丽线虫咽部由胚胎卵裂球阿坝和MS产生。阿坝谱系中的咽部命运由GLP-1/Notch介导的信号和TBX-37和TBX-38 T盒转录因子的组合活性指定。在这里，我们发现另一个T盒因子TBX-2也在ABA衍生的咽部发育中起作用。tbx-2突变体在L1幼虫缺乏大部分或全部ABA衍生的咽肌时停滞。相比之下，tbx-2突变体保留了来源于MS的ABA衍生的边缘细胞和咽肌。线虫胚胎开始接近100细胞阶段。早期表达仅限于少量细胞，其中可能包括ABA衍生的咽前体，而后期表达在体壁肌肉和咽神经元的子集中观察到。TBX-2含有2个共有类小泛素化位点，在酵母双杂交试验中，它与C. elegans SUMO结合途径。ubc-9（RNAi）先前已显示引起可变的胚胎和幼虫停滞，并且我们发现，与tbx-2突变体一样，ubc-9（RNAi）动物缺乏ABA衍生的咽肌。ubc-9（RNAi）也改变了TBX-2：：GFP融合蛋白的亚核分布，表明UBC-9和TBX-2在C.优美的总之，这些结果表明，TBX-2和SUMO结合酶是必需的ABA衍生的咽肌，我们假设，TBX-2功能需要SUMO化。SUMO化越来越多地被认为是控制许多核因子活性的重要机制，这些结果提供了T-box因子活性可能需要SUMO化的第一个证据。(c)2006年爱思唯尔公司All rights reserved.
The C. elegans pharynx is produced from the embryonic blastomeres ABa and MS. Pharyngeal fate in the ABa lineage is specified by the combined activities of GLP-1/Notch-mediated signals and the TBX-37 and TBX-38 T-box transcription factors. Here, we show another T-box factor TBX-2 also functions in ABa-derived pharyngeal development. tbx-2 mutants arrest as L1 larvae lacking most or all ABa-derived pharyngeal muscles. In comparison, tbx-2 mutants retain ABa-derived marginal cells and pharyngeal muscles derived from MS. A tbx-2: :gfp translational fusion is expressed in a dynamic pattern in C. elegans embryos beginning near the 100-cell stage. Early expression is limited to a small number of cells, which likely include the ABa-derived pharyngeal precursors, while later expression is observed in body wall muscles and a subset of pharyngeal neurons. TBX-2 contains 2 consensus sumoylation sites, and it interacts in a yeast two-hybrid assay with the UBC-9 and GEI-17 components of the C. elegans SUMO-conjugating pathway. ubc-9(RNAi) has been previously shown to cause variable embryonic and larval arrest, and we find that, like tbx-2 mutants, ubc-9(RNAi) animals lack ABa-derived pharyngeal muscles. ubc-9(RNAi) also alters the subnuclear distribution of TBX-2: :GFP fusion protein, suggesting that UBC-9 and TBX-2 interact in C. elegans. Together, these results indicate that TBX-2 and SUMO-conjugating enzymes are necessary for ABa-derived pharyngeal muscle, and we hypothesize that TBX-2 function requires sumoylation. Sumoylation is increasingly recognized as an important mechanism controlling activity of many nuclear factors, and these results provide the first evidence that T-box factor activity may require sumoylation. (c) 2006 Elsevier Inc. All rights reserved.