Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7
Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7
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痘苗病毒 K7 蛋白的细胞相互作用组分析确定了三种转运机制作为 K7 的结合伙伴
DOI：
10.1007/s11262-017-1504-5
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发表时间：
2017-08
期刊：
影响因子：
1.6
通讯作者：
Ke YY
中科院分区：
文献类型：
--
作者：
Li Ye;Zhang Leiliang;Ke Youyang;Zhang LL;Ke YY
病毒-宿主相互作用蛋白的鉴定将有助于理解痘病毒如何利用宿主细胞机制。痘苗病毒基因K7R编码一种在大多数正痘病毒中保守的蛋白K7。为了深入了解K7的生物学特性，我们通过GST下拉结合质谱学的方法研究了K7的细胞相互作用组。已确定的顶级蛋白质类别中包含贩运机制的组成部分。为了进一步证实它们与K7的结合能力，我们选择了三种运输机械的关键部件，包括辅助剂、反聚体和雪佛兰。K7的二赖氨酸基序是其与辅酶分子相互作用所必需的，而K7的C末端亮氨酸是逆转聚体结合的关键。我们的研究揭示了痘苗病毒K7的病毒-宿主相互作用组，并揭示了三种宿主运输机制作为K7的结合伙伴，它们可能在痘病毒的生命周期中发挥重要作用。
Identification of viral-host interacting proteins will contribute to understanding of how poxvirus exploits the host cellular machinery. The vaccinia virus gene K7R encodes a conserved protein K7 in most orthopoxviruses. To gain insight into the biology of K7, we investigated the cellular interactome of K7 by GST pulldown coupled with mass spectrometry. The top categories of identified proteins contained components of trafficking machineries. We selected key components of three transport machineries including coatomer, retromer, and CHEVI to further confirm their binding abilities to K7. Di-lysine motif of K7 is required for its interaction with coatomer, while C terminal leucines in K7 are critical for association of retromer. Our study uncovers the viral-host interactome of vaccinia K7 and reveals three host transport machineries as binding partners of K7, which might have important roles in poxvirus’ life cycles.
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DOI：
10.1099/vir.0.052670-0
发表时间：
2013-07
期刊：
The Journal of general virology
影响因子：
--
作者：
Benfield CTO;Ren H;Lucas SJ;Bahsoun B;Smith GL
通讯作者：
Smith GL
影响因子：
4.4
作者：
Zhang, Leiliang;Villa, Nancy Y.;Rahman, Masmudur M.;Smallwood, Sherin;Shattuck, Donna;Neff, Chris;Dufford, Max;Lanchbury, Jerry S.;LaBaer, Joshua;McFadden, Grant
通讯作者：
McFadden, Grant
影响因子：
4
作者：
Seaman, Matthew N. J.
通讯作者：
Seaman, Matthew N. J.
影响因子：
4.4
作者：
Garcia-Dorival, Isabel;Wu, Weining;Hiscox, Julian A.
通讯作者：
Hiscox, Julian A.
影响因子：
4.4
作者：
Garcia-Dorival, Isabel;Wu, Weining;Hiscox, Julian A.
通讯作者：
Hiscox, Julian A.