Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production
Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production
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DOI：
10.1099/jmm.0.001303
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发表时间：
2021-01-01
影响因子：
3
通讯作者：
Into，Takeshi
中科院分区：
文献类型：
--
作者：
Kataoka，Hideo;Mori，Taiki;Into，Takeshi
简介：食物过敏（FA）是由于菌群失调引起的肠道免疫功能障碍而发生的。我们之前确定柠檬酸杆菌在含有 FA 的小鼠粪便中繁殖，并通过诱导过敏性细胞因子 IL-33 加重过敏症状。树突状细胞在FA反应的调节中发挥重要作用。假设。在小鼠肠道中繁殖的柠檬酸杆菌通过刺激树突状细胞诱导IL-33表达而加重过敏症状。目的。本研究的目的是分析C. koseristimulates 树突状细胞以诱导 IL-33 表达。方法。在活的或热灭活的 C 刺激的 DC2.4 小鼠树突状细胞系中评估 IL-33 表达。 koseriJCM1658，从C中提取的ATP、LPS。通过实时 PCR 检测 koseriJCM1658 或其他肠道细菌。同时测量培养物上清液中的ATP浓度和活菌数量。Results.LiveC。 koseriJCM1658 比其他测试的肠杆菌诱导更高水平的 IL-33 表达，但热灭活的 C 不会引起这种反应。科塞里JCM1658。从C中提取的LPS。 koseriJCM1658 不诱导 IL-33 表达并抑制 liveC。 koseriJCM1658 通过激活 Toll 样受体 4 信号传导诱导 IL-33 表达。此外，C 产生 ATP。 koseriJCM1658通过刺激P2X7受体刺激树突状细胞诱导IL-33表达，LPS减弱细胞外ATP诱导的IL-33表达。观察到koseriJCM1658比其他肠杆菌增殖更旺盛，产生更多的ATP。结论。C． koseria 通过产生 ATP 作为一种致敏细菌，刺激树突状细胞诱导 IL-33 表达，同时从失活的 C 中释放 LPS。 koseriJCM1658 可减弱这种过敏性。
Introduction.Food allergies (FAs) occur due to intestinal immune dysfunction elicited by dysbiotic conditions. It was previously determined by us thatCitrobacterspecies propagate in the faeces of mice with FAs and worsen allergic symptoms by inducing the allergenic cytokine IL-33. Dendritic cells can play important roles in regulation of FA responses.Hypothesis.Citrobacterspecies propagating in intestines of mice worsen allergic symptoms by stimulating dendritic cells to induce IL-33 expression.Aim.The aim of the present study was to analyse whetherC. koseristimulates dendritic cells to induce IL-33 expression.Methodology.IL-33 expression was evaluated in a DC2.4 mouse dendritic cell line stimulated by live or heat-inactivatedC. koseriJCM1658, ATP, LPS extracted fromC. koseriJCM1658 or other enterobacteria by real-time PCR. The ATP concentration and number of live bacteria in the culture supernatant were measured simultaneously.Results.LiveC. koseriJCM1658 induced higher levels of IL-33 expression than other enterobacteria tested, but such a response was not elicited by heat-inactivatedC. koseriJCM1658. LPS extracted fromC. koseriJCM1658 did not induce IL-33 expression and suppressed liveC. koseriJCM1658-induced IL-33 expression via the activation of Toll-like receptor 4 signalling. Furthermore, ATP produced byC. koseriJCM1658 stimulated dendritic cells to induce IL-33 expression by stimulating the P2X7receptor, and LPS attenuated extracellular ATP-induced IL-33 expression.C. koseriJCM1658 was observed to proliferate more vigorously and produce more ATP than other enterobacteria.Conclusion.C. koseriacts as an allergenic bacterium through ATP production, stimulating dendritic cells to induce IL-33 expression, while LPS released from inactivatedC. koseriJCM1658 attenuates this allergenicity.