Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex
Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex
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DOI：
10.1016/j.molcel.2019.05.023
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发表时间：
2019-07-25
期刊：
影响因子：
16
通讯作者：
Nasmyth, Kim
中科院分区：
文献类型：
--
作者：
Chapard, Christophe;Jones, Robert;Nasmyth, Kim
粘连蛋白将姐妹 DNA 捕获在 Smc1、Smc3 和 Scc1 之间成对相互作用形成的三联环内。由于 Smc1/3 ATPase 头也可以相互作用，因此粘连蛋白环有可能形成各种子区室。利用体内半胱氨酸交联，我们发现当 Smc1 和 Smc3 ATP 酶在 ATP（E 头）存在的情况下参与时，粘连蛋白环在铰链和 E 头之间产生“SMC (S) 隔室”，并在 E 头和相关 kleisin 亚基之间产生“kleisin (K) 隔室”。 ATP 水解后，粘连蛋白的头部以不同的模式关联，其中它们的标志图案和卷曲线圈紧密并置（J 头），形成替代的 S 和 K 隔室。我们表明E或J类型的K区室可以捕获单个DNA，S期期间Smc3的乙酰化与J头相关，并且姐妹DNA被捕获在J-K区室中。
Cohesin entraps sister DNAs within tripartite rings created by pairwise interactions between Smc1, Smc3, and Scc1. Because Smc1/3 ATPase heads can also interact with each other, cohesin rings have the potential to form a variety of sub-compartments. Using in vivo cysteine cross-linking, we show that when Smc1 and Smc3 ATPases are engaged in the presence of ATP (E heads), cohesin rings generate a "SMC (S) compartment" between hinge and E heads and a "kleisin (K) compartment" between E heads and their associated kleisin subunit. Upon ATP hydrolysis, cohesin's heads associate in a different mode, in which their signature motifs and their coiled coils are closely juxtaposed (J heads), creating alternative S and K compartments. We show that K compartments of either E or J type can entrap single DNAs, that acetylation of Smc3 during S phase is associated with J heads, and that sister DNAs are entrapped in J-K compartments.