HPLC measurement of phenylalanine in plasma.
HPLC measurement of phenylalanine in plasma.
复制标题
血浆中苯丙氨酸的 HPLC 测量。
作者：
N. Atherton;A. Green
苯丙酮尿症 (PKU) 的诊断和随后的饮食管理需要测量血浆中的苯丙氨酸。我们开发了一种等度高效液相色谱 (HPLC) 方法，具有紫外线检测功能。此测量需要 10 微升血浆，完整的分析可在 15 分钟内完成。人血浆样品（苯丙氨酸 136-1541 mumol/L）的批次间变异系数在 2.2% 至 4.4% 之间。该方法具有较宽的线性范围（10-3500 mumol/L），结果 (y) 与离子交换色谱法的结果 (x) 相关性良好（r = 0.996；y = 1.014 x - 11.9；n = 64，范围 22-1823 mumol/L）。仅需要标准 HPLC 设备，不需要专门的样品制备或检测系统。该程序在日常使用中已被证明是稳健且可靠的。
Measurement of phenylalanine in plasma is required for the diagnosis and subsequent dietary management of phenylketonuria (PKU). We have developed an isocratic high-performance liquid-chromatographic (HPLC) method, with ultraviolet detection. For this measurement 10 microL of plasma is needed, and the complete analysis can be done in less than 15 min. Interbatch coefficients of variation for human plasma samples (phenylalanine 136-1541 mumol/L) ranged between 2.2% and 4.4%. The method has a wide linear range (10-3500 mumol/L), and results (y) correlated well with those (x) of an ion-exchange chromatographic method (r = 0.996; y = 1.014 x - 11.9; n = 64, range 22-1823 mumol/L). Only standard HPLC equipment is needed and no specialized sample preparation or detection system is required. The procedure has proved robust and reliable in regular routine use.