A polycomb group protein is retained at specific sites on chromatin in mitosis.
A polycomb group protein is retained at specific sites on chromatin in mitosis.
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在有丝分裂的染色质上，多肉体组蛋白保留在特定位点。
DOI：
10.1371/journal.pgen.1003135
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Francis NJ
中科院分区：
文献类型：
--
作者：
Follmer NE;Wani AH;Francis NJ
基因表达的表观遗传调控，包括多梳蛋白(Polycomb Group，PcG)，可能取决于可遗传的染色质状态，但这些状态如何通过有丝分裂传播尚不清楚。利用免疫荧光和生化分级技术，我们在果蝇S2细胞中发现了与有丝分裂染色体相关的PcG蛋白。有丝分裂细胞染色质免疫沉淀的全基因组测序(CHIP-SEQ)表明，在包括HOX基因在内的有丝分裂中的PcG靶点上，后性梳(PSC)并不存在，但确实存在于间期位点的子集。其中许多持久位点与Sexton等人描述的染色质结构域边界重叠。(2012)，这是以低水平远程接触为特征的基因组区域。持久的PSC结合位点位于两个Hox基因簇的两侧。我们假设，在有丝分裂过程中，长距离染色质接触的中断有助于大多数部位的PcG蛋白释放，而在最少长距离接触的部位的持续结合可能导致有丝分裂后PcG结合和染色体组织的重建。在发育过程中的许多情况下，基因表达谱必须在细胞周期中保持。基因表达谱如何通过有丝分裂由转录调节因子如聚梳组(PcG)蛋白维持还不是很清楚。在这里，我们发现PcG蛋白仍然与有丝分裂染色质有关，整个基因组中的一小部分PcG结合位点在间期和有丝分裂之间保持不变。这些持久结合位点优先与染色质结构域的边界重叠。这些结果提示了一种模型，在该模型中，保留在边界位置的PcG蛋白可能在有丝分裂后使PcG蛋白在区域内重新结合。
Epigenetic regulation of gene expression, including by Polycomb Group (PcG) proteins, may depend on heritable chromatin states, but how these states can be propagated through mitosis is unclear. Using immunofluorescence and biochemical fractionation, we find PcG proteins associated with mitotic chromosomes in Drosophila S2 cells. Genome-wide sequencing of chromatin immunoprecipitations (ChIP–SEQ) from mitotic cells indicates that Posterior Sex Combs (PSC) is not present at well-characterized PcG targets including Hox genes in mitosis, but does remain at a subset of interphase sites. Many of these persistent sites overlap with chromatin domain borders described by Sexton et al. (2012), which are genomic regions characterized by low levels of long range contacts. Persistent PSC binding sites flank both Hox gene clusters. We hypothesize that disruption of long-range chromatin contacts in mitosis contributes to PcG protein release from most sites, while persistent binding at sites with minimal long-range contacts may nucleate re-establishment of PcG binding and chromosome organization after mitosis. Gene expression profiles must be maintained through the cell cycle in many situations during development. How gene expression profiles are maintained through mitosis by transcriptional regulators like the Polycomb Group (PcG) proteins is not well understood. Here we find that PcG proteins remain associated with mitotic chromatin, and a small subset of PcG binding sites throughout the genome is maintained between interphase and mitosis. These persistent binding sites preferentially overlap borders of chromatin domains. These results suggest a model in which PcG proteins retained at border sites may nucleate re-binding of PcG protein within domains after mitosis.
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影响因子：
7.8
作者：
Buchenau, P;Hodgson, J;Strutt, H;Arndt-Jovin, DJ
通讯作者：
Arndt-Jovin, DJ
DOI：
10.1073/pnas.0701538104
发表时间：
2007-10-16
影响因子：
11.1
作者：
Beisel, Christian;Buness, Andreas;Paro, Renato
通讯作者：
Paro, Renato
影响因子：
1.6
作者：
Fanti, Laura;Perrini, Barbara;Pimpinelli, Sergio
通讯作者：
Pimpinelli, Sergio
影响因子：
16
作者：
Blobel GA;Kadauke S;Wang E;Lau AW;Zuber J;Chou MM;Vakoc CR
通讯作者：
Vakoc CR
影响因子：
7
作者：
Enderle, Daniel;Beisel, Christian;Paro, Renato
通讯作者：
Paro, Renato